不同反胶束体系后萃取花生蛋白质的比较

Comparison of the Backward Extraction of Peanut Protein in Different Reverse Micelles

对AOT[二—(2-乙基己基)琥珀酸酯磺酸钠]/异辛烷,SDS(十二烷基硫酸钠)/异辛烷-正辛醇,DTAC(十二烷基三甲基氯化铵)/正庚烷-正己醇3种反胶束体系萃取花生蛋白质的后萃工艺进行研究。主要研究了缓冲溶液pH值、萃取时间、萃取温度、超声功率、KCl浓度对花生蛋白后萃率的影响,分别得到了3种反胶束体系萃取花生蛋白质的最佳后萃工艺条件,并做验证试验。在最优工艺条件下制备不同的花生蛋白样品。通过色差分析,从宏观上比较不同反胶束体系制备的花生蛋白产品色泽的差异,进一步对比不同反胶束体系制备的花生蛋白的扫描电镜(SEM)照片,分析其微观结构的差别,试验结果表明最适合萃取花生蛋白的反胶束体系是AOT反胶束体系,且该体系萃取花生蛋白的后萃率为83.17%,较另外2种体系的后萃率都高。

Peanut protein was extracted using AOT[ sodium bis - （2 -ethylhexyl） sulfosuccinate ] / isooctane, SDS （sodium dodecyl sulfonate） / isooctane- heptylane capryl alcohol, DTAC （heptanes- hexanol reverse micelle systems, and the backward extraction was studied. The effects of pH, extraction time, and temperature, ultrasonic power, KC1 concentration on the backward extraction rates were investigated. And the optimum conditions of the three reverse micelle systems in the backward extraction were obtained and verification test were designed to verify the re- suits. On the optimum conditions, various peanut protein samples were prepared. Through the color difference analy- sis, we compared the color difference of respective peanut protein products on macroscopic. Furthermore, comparing scanning electron microscopy （SEM） pictures of three kinds of peanut proteins extracted from the respective reverse micelle systems, we analyzed the differences of microstructure. The results of the test showed that the most suitable reverse micelle system for the extraction of peanut protein was AOT reverse micelle system; the backward extraction efficiency of this system was 83.17%, which was also higher than the other two reverse micelie systems.