水产饲料用寡肽豆粕制备工艺的研究

Study on Production Technology of Aquaic Feed Oligo-peptide Soybean Meal

为了提高酶解豆粕在水产饲料中的加工质量,以寡肽得率为指标,在单因素试验的基础上采用正交试验优化Alcalase(碱性蛋白酶)和Flavourzyme(风味蛋白酶)同步酶解工艺条件。综合考虑成本和工艺要求等因素,确定双酶法制备寡肽豆粕的最优酶解工艺条件为:底物浓度80 g/L、酶活比(Alcalase:Fla-vourzyme)2∶1、加酶量3 000 U/g、反应pH值8.0、反应温度61℃、酶解时间7.5 h。最终产物寡肽得率为38.16%,DH值为22.69%,产品与!-淀粉具有较佳的亲和性,溶失率为(2.5±0.7)%。结果表明,以寡肽得率为指标,可以有效解决豆粕蛋白质水解进程中蛋白水解过度或不足问题,制备的寡肽豆粕其黏弹性和溶失率能够满足特种水产饲料加工需求。

In order to improve the aquatic feed processing quality of hydrolyzed soybean meal, single factor ex-periment and orthogonal test design were used for optimization of the bi - enzymatic hydrolysis conditions to produce aquatic feed oligo - peptide soybean meal in this study. Based on the evaluation of the production of soybean oligo -peptide, the optimum hydrolysis conditions were designated as substrate concentration of 80 g/L, the ratio of the two enzyme （Alcalase： Flavourzyme） of 2： 1, protease amount of 3 000 U/g, hydrolysis temperature of 61 ℃, pH 8.0 and reaction time of 7.5 h. Finally, a yield of soybean oligo - peptide of 38.16% and a DH of 22.69% were ob- tained. The resultant oligopeptide soybean product had a better processing quality with a greater affinity with - starch and a less leaching loss of product of （2.5 ± 0.7 ） %. The viscoelasticity and leaching losses of oligo - peptide prod- uct were able to meet the processing requirement of aquatic feeds. The results showed that the yield of oligo - peptide was a better indication for controlling the process of soybean meal hydrolysis than the DH （ degree of hydrolysis） to produce a high - quality aquatic feed oligo - peptide soybean meal.