β-谷甾醇与γ-谷维素键合特征的红外光谱分析

Determination of Bonding Forms Between β-Sitosterol and γ-Oryzanol with Fourier Transform Infrared Spectrometer

建立了傅里叶红外光谱法表征β-谷甾醇与γ-谷维素键合特征的方法。在红外图谱中可以看出,β-谷甾醇的羟基伸缩振动峰明显向低波数移动,并导致谱带变宽,γ-谷维素的羰基吸收峰也出现向低波数位移,说明β-谷甾醇与γ-谷维素之间产生了分子间的氢键效应。当β-谷甾醇与γ-谷维素质量比为2∶3时氢键效应最强,并且质构和锥入度分析表明其硬度也最大。β-谷甾醇与γ-谷维素的油脂样品在偏振光显微镜下呈现四瓣形的自组装新晶体。

The bonding forms between β- sitosterol and γ- oryzanol were studied by Fourier transform infrared spectrometer （FTIS）. The stretching vibration of hydroxyl group of β- sitosterol was shifted obviously to the lower wave number as well as the carbonyl group of γ- oryzanol, which indicated the hydrogen bonds in β- sitosterol and γ- oryzanol. The most powerful hydrogen bonds appeared when the ratio of β- sitosterol and γ- oryzanol was 2：3 , and the greatest hardness was also observed by texture analyzer and penetration test. The β- sitosterol and γ-oryza- nol in the oil phase presented a new kind of self - assembly crystal structure with four petal shape, which was ob- served by polarized light microscopy.