胃癌p16、p53和CD_(44)V_6的表达特点及其意义

Expression of p16,p53 and CD_(44)V_6 in Gastric Cancer and Their Significances

目的 探讨p16、p5 3和CD4 4V6在胃癌发生、发展及淋巴结转移的作用机理和意义。方法 用免疫组化ABC法检测p16、p5 3和CD4 4V6在 93例胃癌组织中的表达。结果 93例胃癌中p16、p5 3和CD4 4V6的阳性表达率分别为 39 8%、5 0 5 %、41 9%。p16蛋白在淋巴结转移阳性组的阳性表达率 30 8% ,显著低于淋巴结转移阴性组的阳性表达率 6 0 7% (P <0 .0 1)。p5 3在进展期胃癌、淋巴结转移阳性组的阳性表达率 5 4 9%、5 8 5 % ,显著高于早期胃癌、淋巴结转移阴性组的阳性表达率 18 2 %、32 1% (P <0 .0 5 ,P <0 .0 5 )。CD4 4V6在进展期胃癌、淋巴结转移阳性组的阳性表达率 43 .8%、5 2 .3 % ,显著高于早期胃癌、淋巴结转移阴性组的阳性表达率 9 1%、17 9% (P <0 .0 5 ,P <0 .0 1)。结论 p16蛋白的缺失表达对胃癌的淋巴结转移起重要作用。p5 3、CD4 4V6的高表达对胃癌的浸润、转移起重要作用 ,对分析胃癌的生物学行为和判断预后有重要意义。

Objective To study the expresions of p16、p53 and CD 44 V 6 in gastric cancer and their significances to pathogenesis,infiltration and lymphnode metastasis of this cancer.Methods 93 cases of gastric cancer were detected by using ABC immunohistochemical technique.Results The positive rates of these three expression products were as follows:39.8%(p16),50.5%(p53) and 41.9%(CD 44 V 6).p16 protein expression was correlated with lymphnode metastasis;p53 and CD 44 V 6 expressions were correlated with both clinical staging and lymphnode metastasis.Conclusions p16 protein negative expression is significantly related to the lymphnode metastasis of gastric cancer.The over expression of p53 and CD 44 V 6 may play an important role in predicting the biological behavior and prognosis of gastric cancer.