一种新的ICA亚型及其与胰岛细胞类型关系的免疫组化研究

Immunohistochemical study on ICA subsets and its relation to the islet cellular types

目的 在用免疫组化方法大量检测 ICA基础上发现有弥漫型 ICA和边缘型 ICA两种迥然不同的形态学表现。为探明这两种 ICA所着染的细胞类型及其临床意义 ,进行了以下实验。方法 选取 2 0例弥漫型 ICA和 2 0例边缘型ICA,用免疫组化双标技术鉴定。结果 弥漫型 ICA着染β、α两种细胞 ,边缘型 ICA仅着染α细胞。结论 经初步统计 ,弥漫型 ICA多见于 1型糖尿病 ,2型糖尿病边缘型 ICA比例高于弥漫型 ICA;弥漫型 ICA患者发病年龄比边缘型 ICA患者轻且发病进程快。这种新的 ICA亚型与抗 α细胞抗体的产生以及 α细胞受损有关 ,为进一步探讨 α细胞在糖尿病发病机制中所起的作用提供了一种新的形态学依据。

Objective To explore the changes of the two cellular types,the diffuse pattern and the peripheral pattern,in morphological distribution and their clinical significance.Methods 20 cases from the above each pattern were chosen and identified by double labeling immunohistochemical techniques.Results Both α cells and β cells in pancreatic islet were stained in the diffuse pattern,but only α cells in the peripheral pattern.Conclusion The results from our experiment indicated that the diffuse patterns were more frequently observed in the patients with type I diabetes mellitus and existed in those patients who were more younger at onset and the course of disease developed more rapidly in comparison with the peripheral pattern.The diffuse patterns were more frequent in type I DM,and in type Ⅱ DM the frequence of the peripheral patterns was more higher than that of the diffuse pattern.