铁皮石斛茎段组织培养技术研究

Study on Tissue Culture Techniques for Stem Segment of Dendrobium officinale

以铁皮石斛丛生芽作为外植体,通过研究影响铁皮石斛生长的主要因素,筛选铁皮石斛茎段组培快繁的适宜培养基,总结实用的快繁方法。结果表明,以MS培养基+6-BA 0.5mg/L+NAA 0.2mg/L+30g/L糖+6.5g/L琼脂+0.5g/L活性炭,添加10%的香蕉泥作为天然附加物,pH值5.8,是铁皮石斛丛生芽增殖的最佳培养基;以MS+IBA1.5mg/L+NAA 1.5mg/L为铁皮石斛生根诱导的最佳培养基。通过实验总结出铁皮石斛茎段组培快繁技术,为铁皮石斛的资源保护和应用推广提供重要的科学依据。

The regeneration methods of bud clumps was adopted to establish a rapid technique system of propaga- tion in Dendrobium oftcinale in this experiment. The result showed that the best multiplication medium is MS＋ 6-BA 0.Smg/L＋NAA 0.2mg/L＋30g/L sugar＋6.Sg/L agar＋0.Sg/L activated carbon,add 10% banana mud,pHS.8.And MS＋IBA 1.5mg/L＋NAA 1.5mg/L for the best rooting medium of Dendrohium candidum. This techniques of tissue culture for Dendrobium officinale provided an important scientific basis for resource protection and application.