微卫星不稳定性和杂合性丢失与宫颈癌相关性的研究

The Relationship of MSI and LOH to Cervical Carcinoma and its Clinically Biological Significance

目的 :探讨染色体微卫星不稳定性 (MSI)及杂合性丢失 (lossofheterozygosity ,LOH)在宫颈癌的发生及发展的相关性。方法 :运用p53(D17S786)、9p2 1(D9S171) 2对微卫星标志 ,结合PCR银染技术 ,分别检测 4 8例宫颈癌组织、癌旁组织及周围静脉血DNA的MSI(microsatelliteinstability)和LOH及其与临床分期和病理分级的关系。结果 :4 8例宫颈癌组织的 2个位点进行了微卫星MSI和LOH多态性分析 ,分别为 56%、2 5% ,临床病理分级 (P <0 0 5) ,不同病理分级的统计学分析差异有显著性。结论 :p53(D17S786)、9p2 1(D9S171) 2个不同的位点 17号。

Objective:To elucidate the relationship of MSI and LOH in cervical carcinoma.Melhods:adjacent noncancerous tissues and peripheral vein blood as well as clinical stages and pathological grades were detected using p53(D17S786) and 9p21 (D9S171) as two microsatellite merkers and PCRbased silver staining technology.Results:The microsatellite polymorphism analysis in 48 cases of cervical carcinoma tissues showed that the positive MSI and LOH are 56% and 25% respectively.Clinically pathological grades showed significant difference in statistical analysis.Conclusions:The tumer suppressor gene inactivation on the two different Loci p53 (D17s786) and 9p21 (D9S171) on chromosomes 17 and 19 and MSI may be important factors in the genesis and development of cervical carcinoma.