p16及p15基因在子宫颈癌中的纯合性缺失及其临床意义

Homozygous Deletion of p16 and p15 Genes in Cervical Carcinoma and Clinical Significance

目的 :探讨抑癌基因p16、p15基因的纯合性缺失在子宫颈癌的发生发展中的作用。方法 :应用比较PCR技术检测 34例子宫颈癌组织和 2 0例正常宫颈组织中p16、p15基因的纯合性缺失。结果 :p16、p15基因的纯合性缺失率分别为 11 8%、14 7% ,p16、p15基因的纯合性缺失与组织学类型、临床分期及组织学分级无关。结论 :p16。

Objective:To study the roles of two 2 putative tumor suppressor genes,p16 and p15 genes, in genesis and progression of cervical carcinoma.Methods:Polymerase chain reaction (PCR)based analysis was performed to search for homozygous deletion of the two genes in 34 cervical carcinomas and 20 normal cervical tissues.Results:Homozygous deletion of p16 and p15 genes evaluated by comparative PCR was found in 4 of 34 and 5 of 34 cervical carcinomas respectively while no deletion was detected in corresponding normal tissues. Deletion was not associated with clinical stage and grading.Conclusions:Homozygous deletion of p16 and p15 genes may play an important role in genesis and progression of cervical carcinoma.