Bcl-2、cyclinA2和PI3K在激素受体阴性乳腺癌中的表达及意义

The Expression of Bcl-2,Cyclin A2 and PI3K in Hormone Receptor Negative Breast Cancer and Their Significance

目的探讨激素受体阴性乳腺癌中B淋巴细胞瘤/白血病2基因(B-cell lymphoma/leukemia-2,Bcl-2)、细胞周期素A2(cyclin A2)和磷酸肌醇3激酶(phosphoinositide 3-kinase,PI3K)的表达及意义。方法采用实时聚合酶链反应(realtime polymerase chain reaction,RT-PCR)方法检测Bcl-2、cyclin A2和PI3K在激素受体阴性乳腺癌组织(58例)、乳腺正常组织(14例)中的表达水平,并采用Mann-Whitney U检验分析其与临床病理特征的关系。结果与乳腺正常组织相比,激素受体阴性乳腺癌中Bcl-2表达降低,而cyclin A2和PI3K表达增高(P<0.05)。在乳腺癌组织中,Bcl-2在TNM分期Ⅲ期、组织学分级Ⅲ级和出现转移复发的患者中表达水平降低(P<0.05),但其表达在不同年龄、肿瘤大小和淋巴结转移的组间差异无显著性(P>0.05);cyclin A2在转移复发的患者中显著高表达(P<0.05),但在不同的年龄、肿瘤大小、组织学分级、TNM分期和淋巴结状态的组间差异均无显著性(P>0.05);PI3K在有淋巴结转移和TNM分期Ⅲ期的患者中高表达(P<0.05),但其表达在不同年龄、肿瘤大小、组织学分级以及是否转移复发的组间差异均无显著性(P>0.05)。结论 Bcl-2低表达、cyclin A2和PI3K高表达可能与激素受体阴性乳腺癌的发生发展有关。

Objective To study the expression of Bcl-2, cyclin A2 and PI3K in hormone receptor negative breast cancer and their clinical significance. Methods Bcl-2, cyclin A2 and PI3K amplifications were analyzed using real time PCR in hormone receptor negative breast cancer （58 cases ） and normal breast tissues （14 cases ）. Their relationship with clinicopathological characteristics were analyzed using Mann-Whitney U test. Results Compared with normal breast tissue, the expression of Bcl-2 decreased in hormone receptor negative breast cancer, while the expression of cyclin A2 and PI3K increased （P 〈 0.05 ）. Bcl-2 expression in patients with stage Ⅲ, grade Ⅲ or tumor metastasis group were significantly lower （ P 〈 0.05 ）. But there was no association with age, tumor size and lymph node status （ P 〉 0. 05）. The high expression of cyclin A2 was observed in patients with metastasis or recurrence（ P 〈 0. 05 ）. The expression of cyclin A2 was not significantly different in different groups in terms of age, tumor size, grade, lymph node status and stage（P 〉 0.05）. PI3K expression was significantly higher in the patients with lymph node metastasis or stage Ⅲ （ P 〈 0. 05）. But there was no association with age, tumor size, grade or metastasis （ P 〉 0. 05 ）. Conclusion Bcl-2 low expression, cyclin A2 and PI3K high expression might be closely correlated with the occurrence and development of hormone receptor negative breast cancer.