摘要
Dear Editor, In the past few years, the use of sequence-specific nucle- ases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits. These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining （NHEJ） or homologous recombination （HR） pathways. NHEJ is o^en imprecise and can introduce mutations at tar- get sites resulting in the loss of gene function. In contrast, HR uses a homologous DNA template for repair and can be employed to create site-specific sequence modifications or targeted insertions （Moynahan and Jasin, 2010）.
Dear Editor, In the past few years, the use of sequence-specific nucle- ases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits. These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining （NHEJ） or homologous recombination （HR） pathways. NHEJ is o^en imprecise and can introduce mutations at tar- get sites resulting in the loss of gene function. In contrast, HR uses a homologous DNA template for repair and can be employed to create site-specific sequence modifications or targeted insertions （Moynahan and Jasin, 2010）.
