甘氨脱氧胆酸诱导大鼠肝细胞凋亡

Rat hepatocellular apoptosis induced by glycodeoxycholate

目的探讨甘氨脱氧胆酸（Glycodeoxycholate，GDC）与大鼠肝细胞凋亡的关系。方法以GDC为处理因素，体外培养大鼠原代肝细胞，分别用光镜、电镜、TUNEL原位杂交，流式细胞术和DNA电泳技术观察和分析GDC诱导大鼠肝细胞凋亡的作用。结果大鼠肝细胞在加入终浓度为100μmol／L的GDC培养2h后，光镜、电镜、TUNEL原位杂交，可见凋亡细胞。终浓度为50、100、150μmol／L培养2h，及终浓度为100μmol／LGDC分别培养2、4、6、8hDNA电泳均可见典型Ladder图谱。结论GDC浓度为50，100，150μmol／L能诱导大鼠肝细胞凋亡。

Objective To explore the relationship between glycodeoxycholate (GDC) and rat hepatocellularapoptosis. Methods GDC was used to treat rat hepatocytes cultured in vitro and the apoptotic cells were observed andanalyzed with light microscope, transmission electron microscope, TUNEL in situ hybridization- DNA agarose gel electro-phoresis and flow cytometry. Results When rat hepatocytes were incubated with GDC (final concentration of 100 μmol/L)for 2 h, apoptotic hepatocytes were observed with light and transmission electron microscope, and TUNEL in situhybridization. When the hepatocytes were incubated with GDC (final concentration of 50, 100. 150 μmol/L, respectively)for 2 h, or with GDC (final concentration of 100μ mol/L) for 2. 4. 6. 8 h, respectively, agarose gel electrophoresis of thehepatocytes DNA demonstrated the typical ladder patterns. Conclusion GDC with final concentration of 50, 100, 150μmol/L could induce rat hepatocellular apoptosis.[