有效性确认在交叉配血中的应用

Effectiveness confirming in cross matching quality control

目的:探讨确认交叉配血实验有效性的方法,确保交叉配血实验结果的可控性,保证临床输血安全。方法:在交叉配血实验中用混合A细胞与IgG抗A血清的反应作为阳性对照、自身对照作为阴性对照,用阴阳性对照的实验结果对交叉配血结果是否有效进行确认。结果:所采用的对照物在2009-2011年的516例交叉配血实验中阳性对照均有效,5例阴性对照无效:其中2例由患者在治疗过程中使用肝素导致、1例为新生儿溶血病、2例为自身免疫性溶血性贫血。该5例自身对照出现无效的实验换用其他阴性对照后实验确认为有效;516例患者无一例输血事故发生。结论:在临床交叉配血实验中采用阳性、阴性对照对实验结果的有效性进行确认,可以杜绝实验差错,提高临床输血安全。

Objective：Explore confirm the effectiveness of the crossmatch blood experiment method,insure the controllability in crossmatch,so as to guarantee the safety in clinical transfusion. Method：To affirm the availability by comparison of experimental results form positive-negative-control and outcome from crossmatching.Positive control is by way of feedback from using mixed cell A and IgG anti-A serum in crossmatching of blood,while negative is by auto case-control. Result：The positive controls all remain in force in 516 samples of the adopted contrast in crossmatching between 2009-2011.Invalidation exists in 5 negative samples,including 2 samples are caused by using heparin during therapy,1 sample by hemolytic diseases of newborn,the other 2 are autoimmune hemolytic anemia.When changed to other negative-control,the invalid experiments of auto-case control from the 5 samples turn to valid indeed.No blood transfusion accident has happened of these 516 samples. Conclusion：It can eliminate error in experiments and enhance safety in clinical transfusion through the way of validity affirmation and quality control when adopting results form positive-negative-control in clinical crossmatching.