摘要
目的探讨CD137mAb对宫颈癌患者CIK细胞的功能调节。方法分离宫颈癌患者外周血单个核细胞(PBMCs),常规CIK培养体系中加入CD137mAb为实验组(CD137-CIK组),同时对照组加入鼠IgG1同型对照(IgG1-CIK组)。动态观察CIK细胞体外增殖;流式检测CIK细胞凋亡率、细胞表型及CD3+CD56+细胞内因子的表达;LDH酶释放法检测CIK细胞杀伤活性。结果两组CIK细胞均有很强的增殖活性,实验组细胞浓度最高达(20.92±3.57)×106/mL,对照组为(14.02±2.68)×106/mL(P<0.05);第21天实验组和对照组CD3+CD56+细胞比例分别达到(35.48±5.46)%和(25.12±4.18)%(P<0.05);CD137-CIK组细胞凋亡坏死率明显低于IgG1-CIK组;实验组CIK细胞体外杀伤宫颈癌HeLa细胞株活性明显高于对照组(P<0.05);流式检测CD137-CIK组中CD3+CD56+细胞内IL-2,IFN-γ表达上调,IL-4,IL-10表达下调。结论 CD137mAb介导的共刺激信号可以显著提高宫颈癌患者CIK细胞的体外增殖活性和抗瘤作用。
Objective To investigate the effect of CD137mAb on regulation of CIK cells＇ function of cervical cancer patients.Methods CIK cells were generated by CD3mAb, IL-2, IFN-γ induced from peripheral blood mononuelear cells of cervical cancer patients.Experimental group was added CD137mAb and control group was added mouse IgG1 isotype control, separately.The related phenotypes and the apoptosis of CIK cells and the cytokines production of CD3＋CD56＋celIs were detected by flow cytometry.Cell proliferation was evaluated by cell counting with trypan blue exclusion test.The anti-tumor activity of the CIK cells was determined by LDH Cytotoxicity assay.Results The concentration was respectively arrived at （20.92±3.57） ×106/mL and （14.02±2.68） ×106/mL （P〈0.05）; The flow eytometry analysis showed that the percentage of CD3＋CD56＋ cells increased from （0.48±0.25）% on day 0 to （35.48±5.46）% and （25.12±4.18）% （p〈0.05） on day 21; The rate of apoptosis and necrosis of IgG1-CIK cells was higher than CDt37-CIK. cells; The cells possessed stronger anti-tumor cytotoxic activity in vitro than IgG1-CIK cells; In addition, the production of IFN-γ and IL-2 was increased and the IL-4 and IL-10 was decreased significantly in CD3＋CD56＋cells of CDI37-CIK cells.Conclusion CDl37 signaling could significantly regulate the function of CIK cells from cervical cancer patients, which may provide a new way to treat the cervical cancer.
出处
《中国医药指南》
2013年第18期436-439,共4页
Guide of China Medicine
