BMI-1基因在骨肉瘤中的表达及其临床意义

The expression of BMI-1 gene in osteosarcoma and its clinical significances

背景:目前,骨肉瘤发病机制的研究已逐步深入。BMI-1基因作为近年来肿瘤致癌基因研究中的热门分子,在恶性肿瘤方面的研究证据较为丰富,但针对其在骨肿瘤方面的研究相对较少。目的:探讨BMI-1基因在骨肿瘤中的表达及其临床相关性研究。方法:采用免疫组化染色检测80例骨肿瘤组织及20例非肿瘤骨组织中BMI-1蛋白的表达,骨肿瘤包括骨肉瘤30例(分为EnnekingⅠ、Ⅱ、Ⅲ期)、骨软骨瘤20例、软骨肉瘤20例、尤因肉瘤10例。并对30例骨肉瘤的主要临床资料进行比较,用卡方检验进行统计学处理。结果:BMI-1基因在骨肉瘤、软骨肉瘤及尤因肉瘤中均有较高的阳性表达率,相比较无统计学差异;而在骨软骨瘤和非肿瘤骨组织中阳性表达率明显较低,且存在统计学差异(P=0.000);良、恶性骨肿瘤之间的阳性表达率亦存在统计学差异(P=0.000)。骨肉瘤EnnekingⅠ、Ⅱ、Ⅲ期之间两两比较BMI-1蛋白的阳性表达率无统计学差异;不同年龄、性别、肿瘤发生部位及术后是否有转移中BMI-1蛋白的阳性表达率均无统计学差异,但年龄越小,BMI-1蛋白的阳性表达率越高以及有转移骨肉瘤患者的BMI-1蛋白阳性表达率升高。结论:BMI-1基因可能为骨肉瘤的分子靶点之一,并具有作为判断骨肉瘤进展及患者预后参考指标的潜力。揭示其作用于骨肉瘤的分子机理,将有助于骨肉瘤发病机制的探索。

Background：Nowadays,the research of the pathogenesis of osteosarcoma is becoming intensive.BMI-1 gene,as a popular element in the research of carcinogene,has a lot of research evidences in the recent malignant tumors studies.However,it has few evidences in the osteosarcoma research comparatively.Objective：To explore the expressions of BMI-1 gene in bone tumors and their clinical correlations.Methods：Adopting immunohistochemical staining to detect the expression of BMI-1 protein in 80 cases of bone tumor tissues and 20 cases of non-tumorous bone tissues.Of the 80 cases of bone tumor,there were 30 cases of osteosarcoma（including Enneking stage I,II,and III）,20 cases of osteochondroma,another 20 cases of chondrosarcoma,and 10 cases of Ewing＇s sarcoma.Then compare the main clinical data within the osteosarcoma cases,and use the Chi-square test in its statistical processing.Results：The positive expression rates of the BMI-1 protein were all relatively high in the osteosarcoma,chondrosarcoma and Ewing＇s sarcoma cases,there were no statistical differences between them.While that rates were significantly low in the osteochondroma and non-tumorous bone tissues cases,there existed statistical differences.The statistical differences also existed in the positive expression rates of the benign bone tumors and the malignant bone tumors.While there were no statistical differences between each two of the positive expression rates of BMI-1 protein in the Enneking I,II,III stage of osteosarcoma;Nor statistical differences among different ages,sexes,tumor locations or postoperative metastasis.But a higher positive expression rate of BMI-1 protein was more likely to be found in younger patients,and the rise of the that rate could predict a tendency of the osteosarcoma metastasis.Conclusions：BMI-1 gene can be one of the molecular targets of the osteosarcoma,it has the potential to be a reference index in judging the developing of the osteosarcoma and its prognosis.Revealing its molecular mechanism in osteosarcoma will be helpful in explore the pathogenesis of osteosarcoma.