轻度修饰低密度脂蛋白对血管内皮细胞粘附功能影响及其机理初探

Effect of mininally modified low density lipoprotein on the adhesive function of vascular endothelial cell and its mechanism

目的 :观察轻度修饰LDL (MM -LDL)对培养人脐静脉内皮细胞 (HUVEC)与人类单核细胞系U937粘附功能及其表面粘附分子表达的影响。方法 :利用计数法观察经MM -LDL作用的HUVEC与U937细胞的粘附率 ;用ELISA方法检测MM -LDL作用后HUVEC膜表面粘附分子血管细胞粘附分子 - 1(VCAM - 1)、细胞间粘附分子 - 1(ICAM - 1)及P选择素 (P -selectin)的表达。结果 :MM -LDL(75mg/L)作用HUVEC 4h后 ,其对U937细胞粘附率明显增加 (P <0 0 1) ,HUVEC膜表面未见VCAM - 1,ICAM - 1,P -selectin表达上调 ,作为阳性对照重组肿瘤坏死因子α(rTNFα) 5 0 μg/L可显著诱导以上 3种粘附分子表达。延长MM -LDL与HUVEC作用时间至 18h可诱导产生P -selectin表达 ,对VCAM - 1表达无影响。结论 :MM -LDL诱导的HUVEC与U937粘附不是通过ICAM - 1、VCAM - 1介导的 ,P

AIM: To observe the effect of MM-LDL (minimally modified LDL) on the interaction between human umbilical vein endothelial cell (HUVEC) and U937 monocyte-like cell line and the exporession of vascular cell adhesion-1 (VCAM-1), intercellular adhesion molecule-1(ICAM-1), P-selectin.METHODS:The adhesive percentage between HUVEC treated with MM-LDL and U937 was determined by counting and the expression of VCAM-1, ICAM-1, P-selectin were examined by ELISA. RESULTS: Treatment of HUVEC with MM-LDL (75 mg/L) for 4 hours significantly increased adhesion of U937 to HUVEC ( P <0 01) and did not induce the surface expression of VCAM-1, ICAM-1, P-selectin . Recombination tumor necrosis factor-alpha (rTNF α) 5 0 μg/L, as a positive control, induced the expression of these adhesion molecules ( P <0.05). Prolonged (18 h) exposure to MM-LDL resulted in the expression of P-selectin, but not VCAM-1. CONCLUSION: the adhesion of monocytes to endothelial cells induced by MM-LDL is not mediated by VCAM-1, ICAM-1. P-selectin induction may be partly involved in the process.