谷氨酰胺缺乏对急性早幼粒白血病原代细胞生长和分化的影响

Effect of glutamine deprivation on growth and differentiation of primary APL cells

目的 :了解谷氨酰胺 (Gln)缺乏对急性早幼粒白血病 (APL)原代细胞生长和分化的影响。方法 :从 18例未经治疗的APL病人外周血中提取APL原代细胞 ,在无Gln的RPMI 16 40培养液中补加 10 %胎牛血清 ,以活细胞密度 5× 10 8/L接种细胞 ,在 37℃ ,5 %CO2 和饱和湿度下培养 4d ,计数活细胞 ,并收集细胞行Wright-Giemsa、DNA、POX、NAE及NaF抑制试验、墨汁吞噬试验和NBT还原试验等细胞化学染色 ,于油镜下观察。结果 :经 4d培养 ,其活细胞密度为起始活细胞密度的 (5 4 2 8± 4 2 8) % ,而对照组为 (10 8 5 6± 12 2 7) % (P <0 0 1) ;成熟分叶核粒细胞和杆状粒细胞比例显著高于对照组 (P <0 0 1)。结论 :缺乏Gln可使APL原代细胞生长受到抑制 。

AIM:To investigate the effect of Gln deprivation on growth and differentiation of primary APL cells. METHODS: The primary APL cells were collected from 18 cases of APL patients' periphery blood, the patients had not been treated. The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10% fetal calf sera, at 37℃,5% CO 2 and saturation humidity for 4 days.The initial living cell density was 5×10 8/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens. RESULTS: The living cell density became (54.28±4.28)% of the initial in glutamine deprivation group, but the living cell density in control with 4 mmol/L Gln was (108.56±12.27 )% of the initial ( P <0.01) after 4 days incubation. The cells differentiated into mature granulocytes in glutamine deprivation group. CONCLUSION: Deprivation of glutamine inhibited primary APL cell growth and induced cell differentiation toward mature granulocyte.