摘要
本试验通过PCR-RFLP技术对大白猪、长白猪和杜洛克猪的催乳素受体(prolactin receptor,PRLR)基因第10外显子AluⅠ酶切位点进行多态性分析,并对各基因型母猪的第1至第3胎次产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄窝重及断奶成活率进行测定,进而分析PRLR基因多态性与繁殖性能的关系。结果表明,PRLR基因在3个品种中均存在多态位点,且AA基因型都是其优势基因型。关联分析结果表明,大白猪AA基因型个体第1、2和3胎各繁殖性状均呈现出高于AB和BB基因型的趋势。在繁殖性能已经稳定的第3胎次AA基因型个体产仔数、产活仔数、断奶仔猪数、初生窝重及断奶成活率比BB基因型个体平均分别高出0.31头、0.63头、0.02头、0.65kg和0.43%,但差异均不显著(P>0.05)。
The polymorphisms of Alu I site at extron 10 of prolactin receptor (PRLR) gene in Yorkshire, Landrace and Duroc populations from Taiwan were detected with PCR-RFLP method, meanwhile the total number born (TNB), number born alive (NBA), number of alive weaning litter (NW), body weight of litter (BWL), weaning weight of litter (WWL) and weaning survive rate of different genotypes from the first to the third parity were measured, and then the relationship between reproductive performance and different genotypes were analyzed in this article. The results showed that the polymorphic sites of PRLR gene were found in all three breeds, in addition, the dominant genotype was AA genotype in Yorkshire, Landraee and Duroc. Relativity analysis indicated that reproductive trait of AA genotype of Yorkshire from the first to the third parity was on the whole higher compared with AB and BB genotypes. AA genotype of Yorkshire in the third parity produced 0.31 TNB, 0.63 NBA, 0.02 NW, 0.65 kg BWL and 0.43~ weaning survive rate more than BB genotype, while there was no significant difference between them.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第8期146-150,共5页
China Animal Husbandry & Veterinary Medicine
基金
江苏省科技支撑计划(农业)(BE2010371
BE2012330)
扬州市农业科技攻关项目(YZ2011048
YZ2011153)
江苏省研究生科研创新计划(CXLX12-0927)
关键词
催乳素受体基因
繁殖性能
大白猪
长白猪
杜洛克猪
prolactin receptor gene
reproductive performance ~ Yorkshire
Landrace
Duroc