三七对酒精肝大鼠肝组织TGF-β1/Smads,CTGF表达量的影响

Effect of Notoginseng Radix on expression quantity of TGF-β1/Smads and CTGF mRNA in rats with alcoholic liver disease

目的:研究三七对酒精性肝损害大鼠肝脏TGF-β1/Smads,CTGF的影响,探讨三七对酒精性肝硬化的保护作用机制。方法:50只SD大鼠随机分成正常对照组、模型组、三七高、低剂量组、异甘草酸镁组,除对照组外大鼠灌服白酒-玉米油-吡唑混合液14周建立酒精性肝病模型,造模同时三七高、低剂量组分别灌服12,3 g·kg-1,异甘草酸镁组腹腔注射24 mg·kg-1,每天给药1次,连续14周。测定肝功能、大鼠肝脏Masson染色病理学检查,以及各组大鼠肝组织TGF-β1,Smad3,Smad7,CTGF mRNA的表达量。结果:与模型组比较,治疗组能明显降低肝脏ALT,AST,抑制胶原纤维沉积,减少肝组织TGF-β1,Smad3,CTGF mRNA的表达量,增加Smad7 mRNA的表达量,三七组不同剂量组之间存在显著性差异,高剂量三七组与异甘草酸镁组之间无显著性差异。结论:三七可以影响TGF-β1/Smads信号通路,减少CTGF表达。

Objective： To evaluate the effect of Notoginseng Radix on hepatic expression of transforming growth factor β1 （TGF-β1） and connective tissue growth factor （CTGF） in rats with alcoholic liver disease （ALD） , in order to discuss its protective effect on alcoholic cirrhosis. Method： Fifty SD male rats were divided into the normal control group, the model group, the high-dose and low-dose Notoginseng Radix groups （3.0,12. 0 g · kg^-1 ） and the magnesium isoglycyrrhizinate group （24 mg·kg^-1 ）, with 10 rats in each group. Apart from the control group, other groups were administered with ethanol-cornoil-pyrazole for 14 weeks to establish the alcoholic liver disease model. During the establishment of the model, the high-dose and low-dose Notoginseng Radix groups were ad- ministered with 12 g · kg^-1 · d^-1 Notoginseng Radix for 14 weeks, once everyday. Efforts were made to detect liver function, pathology with Masson staining, and the expressions of TGF-β1, Smad3, Smad7 and CTGF mRNA. Result： Compared with the rats in model group, rats in Notoginseng Radix groups showed significant reduction in liver ALT, AST, collagen fiber deposition, and TGF-β1, Smad3 and CTGF m RNA expressions in liver tissues, with the increase in the expression quantity of Smad7 mRNA. There were differ-ences between the Nntoginseng Radix groups. No significant difference was observed between the high-dose Notoginseng Radix group and the magnesium isoglycyrrhizinate group. Conclusion： Notoginseng Radix can affect TGF-β1/Smads signaling pathway and reduce the expression of CTGF.