利用单克隆抗体特异性敲除技术解析中药药效物质基础的新方法

New method for analyzing pharmacodynamic material basis of traditional Chinese medicines by using specific knockout technology with monoclonal antibodies

中药药效物质基础研究是实现中药现代化的关键问题之一。作者将小分子单克隆抗体技术引入中药药效物质基础研究,利用中药活性成分的单克隆抗体制备相应的免疫亲和色谱柱,实现从药材或复方中特异性地"敲除"该化合物,而"原样"保留其他成分在量和比例关系上不变,通过药理药效的比较研究,来明确揭示该成分与整体中药或复方功能主治的关联度。利用单克隆抗体特异性敲除技术解析中药药效物质基础,是符合中药自身特点的药效物质基础研究新方法,获得的研究结果不仅有助于从全新的角度理解物质基础,而且有助于理解中药单味药内部,或复方药味之间"配伍"的现代科学意义。

Study on pharmacodynamic material basis of traditional Chinese medicines is one of the key issues for the moderni-zation of traditional Chinese medicine. Having introduced the monoclonal antibody technology into the study on pharmacodynamic mate-rial basis of traditional Chinese medicines, the author prepared the immunoaffinity chromatography column by using monoclonal anti-bodies in active components of traditional Chinese medicines, so as to selectively knock out the component from herbs or traditional Chinese medicine compounds, while preserving all of the other components and keeping their amount and ratio unchanged. A compara-tive study on pharmacokinetics and pharmacodynamics was made to explicitly reveal the correlation between the component and the main purpose of traditional Chinese medicines and compounds. The analysis on pharmacodynamic material basis of traditional Chinese medi-cines by using specific knockout technology with monoclonal antibodies is a new method for study pharmacodynamic material basis in line with the characteristics of traditional Chinese medicines. Its results can not only help study material basis from a new perspective, but also help find the modem scientific significance in single herb or among compounds of traditional Chinese medicines.