湖羊PRLR基因遗传多样性及其与母性行为的关联分析

Polymorphisms of the PRLR Gene in Hu Sheep and Its Association Analysis with Maternal Behaviours

本研究运用PCR-SSCP技术,分析催乳素受体(PRLR)基因外显子10序列的多态性,旨在研究PRLR基因与母羊母性行为关联性。结果表明:仅引物3扩增片段具有多态性;群体内遗传多样性分析表明,基因型AA、AB和BB符合Hardy-Weinberg平衡,表明该群体可进行相关性状的选育;不同基因型和母性行为性状进行最小二乘分析结果表明,AA型和AB型个体在舔舐和踩踏行为的观察数间差异不显著(P>0.05),但均与BB型个体的差异显著(P<0.05);哺乳行为则在AA、AB和BB 3种基因型间依次降低,且差异均显著(P<0.05);拒绝哺乳行为在各基因型个体间差异不显著(P>0.05)。因此,可推测等位基因A可能为优势基因。

Good maternal behavior is critical to the survival of the correlation between the polymorphism of prolactin receptor baby animals. The purpose of this study was to explore （PRLR） gene and maternal behavior. The PCR-SSCP technique was applied to study the polymorphism of PRLR gene exonl0.The results showed that only the products amplified by primer 3 displayed polymorphisms and three genotypes （AA, AB and BB） were detected. The analysis of genetic diversity level within populations indicated that three genotypes were accorded with Hardy-Weinberg equilibrium, which indicated that the populations could be selecting for maternal traits. Using least square analysis, it was observed that there was no significant difference between observations for licking and trampling behavior of AA and AB genotypes individuals （P〉0.05）, but it had significant difference compared with BB genotype （P〈0.05）. However, for nursing behavior, the observations of the three genotypes were decreased gradually, and there was significant difference （P〈0.05）. No obvious difference was found in three genotypes for the behavior of refusing to feed young sheep （P〉0.05）. Consequently, we conjectured that the allele A might be an advantageous gene.