石化废水中常见有机酸的液相色谱分析

Analysis of Common Organic Acids in Petrochemical Wastewater by High Performance Liquid Chromatography

利用高效液相色谱（HPLC）法同时检测石化废水中丙烯酸、对甲基苯磺酸、甲基丙烯酸。水样调节pH=1．6，经0．45μm醋酸纤维滤膜过滤后直接进行色谱分析。色谱条件：流动相为体积比93：7的磷酸盐缓冲溶液（pH=3）和乙腈，流速1．0mL／min，ZORBAX-SB—C18色谱柱，柱温30℃，MWD检测器，检测波长195nm，进样量10μL。丙烯酸、对甲基苯磺酸、甲基丙烯酸的最低检出限分别为0．030、0．028和0．050mg／L；样品测定的相对标准偏差分别为1．83％、0．69％和0．84％；样品加标回收率96％～112％。

High performance liquid chromatography（HPLC） was used for simultaneous detecting acrylic acid, methacrylic acid and p-toluenesulfonic acid in petrochemical wastewater. The pH of the water sample was adjusted to 1.6, and then solution was filtered with 0. 45μm cellulose acetate membrane before chromatographic analysis. The optimized chromatographic conditions are as follows： phosphate buffer solution （ pH = 3.0）/acetonitrile （ volume ratio is 93：7 ） as the mobile phase, flow rate = 1.0 mL/min, ZORBAX-SB- C18 column, temperature = 30℃, MWD detector with detection wavelength at 195 nm, and the injection volume of 10μL. Under the optimal conditions, the detection limit of acrylic acid, p-toluenesulfonic acid and methacrylic acid is 0. 030, 0. 028 and 0. 050 mg/L, respectively. The relative standard deviations of the samples are 1.83%, 0. 69% and 0. 84%, respectively, while the recoveries are in the range from 96% to 112%.