混合酸在金银花指纹图谱研究中的应用

Application of Mixed Acid in Fingerprint of Lonicera japonica Thunb.

目的：建立金银花指纹图谱混合酸流动相测定方法。方法：采用高效液相色谱法,色谱柱为DIKMADiamon-silC18（2）色谱柱（4.6mm×250mm,5μm）,以乙腈为流动相A,以含0.025%磷酸、0.3%醋酸的溶液为流动相B,进行梯度洗脱;检测波长为243nm。柱温为40℃。结果：使用混合酸能改善30～50min各色谱峰的分离度,使52min混合峰完全基线分离。结论：混合酸流动相使金银花色谱峰分离度增加,可用于金银花指纹图谱的研究。

Objective ： To establish a HPLC mixed acid mobile phase method for determination of the fingerprint of Lonicera ja-ponica Thunb. Methods ：A Diamonsil C18 （2）column（4.6 mm × 250 mm, 5 μm） was used, acetonitrile as the mobile phase A, a mixed solution of 0. 025% phosphoric acid,0. 3% acetic acid as the mobile phase B, gradient elution, detective wavelength was 243 nm and column temperature was 40 ℃. Results ： The mixed acid mobile phase can improve separation 30 to 50 minutes chrom-atographic perks, making the mixed peaks of 52 minutes complete baseline separation. Conclusion：The mixed acid mobile phase can increase separation and suitability for the determination of the fingerprint of Lonicera japonica Thunb.