摘要
目的:研究紫草素诱导体外培养的子宫内膜癌Ishikawa细胞凋亡作用及其机制。方法:流式细胞术分析细胞周期分布及凋亡率变化;免疫组化法(SABC法)检测Bcl-2、Bax蛋白的表达。结果:紫草素可诱导细胞凋亡,改变细胞周期分布,多数细胞周期阻滞于G1期,细胞凋亡率差异有统计学意义(P<0.05);紫草素可上调Bax和下调Bcl-2基因蛋白的表达,与空白组对照差异有统计学意义(P<0.05)。结论:紫草素能够诱导子宫内膜癌Ishikawa细胞凋亡,其凋亡机制可能与促进Ishikawa细胞中Bax上调,Bcl-2下调相关。
Objective : To investigate apoptosis effects and mechanisms of Shikonin on human endometrial carcinoma cell line - Ishikawa. Methods : Evaluation of cell cycle and apoptosis was ascertained respectively, which used trypan blue exclusion and flow cytometry. The protein expression of Bcl-2 and Bax was detected by immunohistochemical methods. Results-Shikonin could induce the apoptosis of Ishikawa cells, change cell cycle percentage and block cell cycle in G1. There was significant difference in the ap- optotic percent of cell (P 〈 0.05 ) ; Shikonin can up-regulate gene expression of Bax and down - regulate gene expression of Bcl-2. There was significant difference between Shikonin groups and control group( P 〈 0. 05 ). Conclusion:Shikonin could promote apop-tosis in Ishikawa cells. The mechanism may be associated with the gene expression of up - regulation of Bax and inhibition of Bcl-2.
出处
《辽宁中医杂志》
CAS
2013年第9期1930-1932,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
上海市卫生三年行动计划海派基地建设项目(ZYSNXD-CC-HPGC-JD-008)