高效液相色谱法测定茶饮料中的维生素C

Determination of Vitamin C in Tea Beverages by High Performance Liquid Chromatography

建立了茶饮料中维生素C含量的高效液相色谱检测方法，采用ZOBRAX SB-C18色谱柱，以0．1％醋酸溶液作流动相，流速1．0mL／min，柱温28℃，二极管阵列检测器，检测波长250nm。结果表明，在10．0～300mg／L浓度范围内，浓度与仪器响应值成线性关系，相关系数r=0．9999（n=7），加标回收率为99．7％～102％，测定四份不同茶饮料的相对标准偏差RSD分别为1．30、0．50、0．92、1．21％，检出限为0．009mg／L。该法快速、稳定、无有毒有害试剂，能准确测定茶饮料中维生素C的含量，符合绿色化学发展方向。

A method was established for determining Vitamin C （Vc） in tea beverages by high performance liquid chromatography （HPLC）. The determination was conducted with a ZOBRAX SB-C18 column used at 28℃. The mobile phase was 0.1% acetic acid solution, a DAD detector was used with a flow rate of 1.0mL/min and a detection wavelength of 250nm. The standard working curve of Vitamin C had a good liner relation in the concentration range from 10 to 300mg/L, and the relative coefficient was 0.9999 （n = 7）. The recoveries were 99.7% - 102%, the RSD were 1.30, 0.50, 0.92 and 1.21%, respectively, and the detection limit 0.009mg/L. Complying with green chemistry, the method was an accurate and rapid way to determine the content of Vitamin C in tea beverages without toxic and harmful reagents.