不同益气活血中药组方对心衰大鼠MMP-9影响的实验研究

Experiment Research on Effect of Different Chinese Medicine Compound on MMP-9 Expressions in Rats with Chronic Heart Failure

目的:通过研究基质金属蛋白酶(MMP-9)在慢性心衰中的作用,从分子生物学角度探讨不同益气活血中药组方对慢性心衰(MMP-9)及心室重构的影响。方法:本实验以冠脉结扎法配合力竭式游泳、减食等方法造成大鼠慢性心衰动物模型,分成模型组、西药组、益气组、活血组、益气活血组和正常组。利用实时荧光定量PCR技术及SABC免疫组化法检测慢性心衰大鼠MMP-9的变化情况。结果:慢性心衰动物模型组大鼠心肌MMP-9表达明显升高,与模型组比较,差异有统计学意义(P<0.01);经过治疗,各用药组大鼠心肌组织MMP-9表达明显降低,其中,西药组、益气组、活血组各组间比较,差异无统计学意义(P>0.05),益气活血组疗效明显优于益气组、活血组(P<0.05)。结论:证明益气活血中药组方对心衰的疗效显著,并揭示其可通过作用于心肌组织中MMP-9的表达,抑制或逆转心室重构的过程,从而达到治疗慢性心衰的目的。

Objective：By investigating the effects of different Chinese medicine compounds on expressions of matrix metal-lopeptidas 9（MMP-9）in chronic heart failure（CHF）,we studied the effect of traditional Chinese medicine on further mechanism of ventricular remodeling in CHF on the role of molecular biological sense.Methods：The coronary artery ligation method with exhaustion swimming and reduce feeding was used to cause chronic heart failure rats model.Rats were divided into model group,Western medicine control group,Qi-supplementing group,blood-activating group,Qi-supplementing and blood-activating group and normal group.The real-time quantitative PCR technique and SABC immunohistochemical method were used to detect MMP-9 changes of CHF rats.Results：MMP-9 expression in animal model of chronic heart failure group was increased obviously.The difference in the Qi-supplementing group and blood-activating group and Western medicine control group were not statistically significant（P〈0.05）.And therapeutic effect of Qi-supplementing and blood-activating group was much better than that in the Qi-supplementing group and blood-activating group（P〈0.05）.Conclusion：It is proved Chinese compound of supplementing Qi,activating blood circulation and promoting urination can inhibit or reverse ventricular remodeling process by acting on MMP-9 in organizations,so as to achieve the goal of therapy for chronic heart failure.