健脾疏肝法、活血化瘀法对肝纤维化小鼠肝组织TIMP-1及血清纤维化指标的影响

Effect on Serum Markers of Liver Fibrosis and TIMP-1 in Liver Tissue of Liver Fibrosis Rats Treated withShugan Jianpi Recipe andHuoxue Huayu Recipe

目的:观察健脾疏肝法、活血化瘀法分别对肝纤维化小鼠肝组织TIMP-1及血清纤维化指标的影响。方法:选雄性Wistar小鼠75只,随机分为正常对照组、模型对照组、健脾疏肝组、活血化瘀组、秋水仙碱组各15只,除正常对照组外,各组采用40%CCL4橄榄油腹腔注射诱导小鼠肝纤维化。空白对照组15只,腹腔注射0.9%生理盐水0.5 mL,给药方法及时间同上。造模后第5周,各组给予不同药物干预(10mL/kg),正常对照组和模型对照组给予0.9%生理盐水10 mL/kg体重灌胃;健脾疏肝组予健脾疏肝方;活血化瘀组予复方鳖甲软肝片;秋水仙碱组给予秋水仙碱。8周后处死动物取肝组织,检测肝脏组织中的TIMP-1、血清纤维化指标。结果:活血化瘀组、健脾疏肝组、秋水仙碱组小鼠血清纤维化指标明显低于模型对照组(P<0.05),其中健脾疏肝组优于其他两组。并且活血化瘀组、健脾疏肝组、秋水仙碱组大鼠肝组织中TIMP-1均低于模型对照组(P<0.05),其中健脾疏肝组优于其他两组。结论:疏肝健脾法能够有效的治疗肝纤维化,其机制可能与抑制肝脏组织中的TIMP-1的表达有关。

Objective ：To observe the effect on serum markers of liver fibrosis and TIMP-1 in the liver tissue of liver fibrosis rats which are given Shugan Jianpi Recipe.75 male rats were devided into five groups randomly ：control group,model group,Jianpi Shugan group,Huoxue Huayu group,colchicine group.The rats in each group were injiected pertoneally with 40% carbon tetrachloride olive oil to induce liver fibrosis expect control group.The rats in the control group were injected peritoneally with 0.9% physically saline 0.5 mL.Each group were intervented with different drugs from the fifth week.The rats in the model group and the control group were given the gastric lavage of 0.9% physical saline 10 mL/kg.Jianpi Shugan group were given the Jianpi Shugan presciption;Huoxue Huayu group were given the compounded Biejia Ruangan tablet.The colchicine group were given the colchicine.The rats were put to death after 8 week,testing the TIMP-1 in the liver tissue and the fibrosis marker in the serum.Rusult ：The liver fibrosis markers in the serum of the other three groups were lower than that in the model group（P〈0.05）.TheJianpi Shugan group was better than the other two groups.The TIMP-1 in the liver tissue of the other three groups were lower than that in the model group（P〈0.05）.The Jianpi Shugan group was better than the other two groups.Conclusion ：Jianpi Shugan prescription can significantly treat liver fibrosis,and reducing the expression of TIMP-1 in the liver tissue may be the mechanism.