脾阳虚证大鼠回肠水通道蛋白4表达变化研究

Expression of Aquaporin 4 in Ileum of Spleen Yang Deficiency Rats

目的:通过对脾阳虚大鼠回肠水通道蛋白4(AQP4)表达的检测,探讨脾阳虚证状态下大鼠水液代谢的影响。方法:对正常组、脾阳虚模型组、中药反证组分别行免疫组织化学、RT-PCR及Western blot的方法检测AQP4在回肠的表达及分布情况。结果:①脾阳虚状态下,大鼠回肠上皮细胞细胞膜的AQP4分布量显著减少,经温补脾阳中药治疗后,其分布量有所回升,但仍低于正常组。②脾阳虚模型组AQP4 mRNA及蛋白表达量均明显低于正常组(P<0.05)。中药反证组AQP4 mRNA表达量均明显高于模型组(P<0.05),蛋白表达虽然有所提高,但却没有统计学意义(P>0.05)。结论:脾阳虚证状态下大鼠回肠AQP4表达下调,导致回肠对水分的吸收出现障碍,提示可能与脾阳虚证的病理机制有关。

Objective：To investigate the expression and distribution of aquaporin 4（AQP4）in ileum of the spleen Yin deficiency rats,and to discuss the relationship between AQP4 expression and ileum water metabolism.Methods：Reverse transcription-polymerase chain reaction（RT-PCR）and Western blot were used to detect the expression of AQP4 mRNA and protien on the ileum in this study.Results：（1）AQP4 mainly expressed in cells menbrane.（2）The expression of AQP4 mRNA and protien in the model group were significantly down-regulated than those in the control group（P〈0.05）.The expression of AQP4 mRNA in the treatment group was significantly up-regulated than that in the model group（P〈0.05）.The expression of AQP4 protien in the treatment group was more obviously up-regulated than that in the model group,but it had no statistical significance（P〈0.05）.Conclusion：AQP4 was down-regulated in rats ileum.This may lead to decreased water reaborption by the ileum.As a result,AQP4 may be one of the therapeutic mechanism of spleen Yin deficiency.