动物模型行为实验对“肾虚致恐”的证明

Kidney deficiency resulting in fear stress： an evidence from behavior experiment on animal model

目的通过行为实验中动物模型的表现差异，分析生理因素“肾虚”与心理应激状态“惊恐”的相关性。方法以肌肉注射氢化可的松建立肾阳虚大鼠模型作为实验组（以下简称“肾阳虚组”），以猪油加生理盐水灌胃建立脾阳虚大鼠模型作为控制条件对照（以下简称“脾阳虚组”），以正常大鼠作为空白对照组（以下简称正常组），将光线、高温热源作为惊恐刺激条件的变量，将食物作为环境刺激条件的变量；分析各组大鼠在行为实验中的表现差异，比较三种不同的生理状态作为影响因素与惊恐应激反应之间的相关性。结果行为实验中，各组对于惊恐刺激的敏感性差别较大，组间差异具有统计学上的显著性（在设置食物刺激前后分别为P=0．039、P=0．037。如在无食物，仅光线刺激条件下，15min内平均探身次数空白对照组为（5．70±1．636）次、脾阳虚组为（2．30±0.949）次、肾阳虚组为（0．05±0．224）次。与正常组相比，肾阳虚组、脾阳虚组探身次数较少，表现出明显的惊恐特征。其中，肾阳虚组探身次数最少，惊恐特征最明显。结论肾虚是导致惊恐应激反应的显著因素，二者之间可能具有直接相关性。

Objective To analyze the correlation between kidney deficiency and fear stress by investigating the difference in behavior between groups of model rats. Methods The rats were divided randomly into 3 groups. To create kidney deficiency models as the experimental group by intramuscular injection of hydrocortisone; giving high-fat and high-salt diet by gastric perfusion to create spleen deficiency models as the control group; normal rats were used as the blank control group. Using light stimulation, thermo stimulation and food stimulation as the factors, to observe and analyze the difference of behavior among groups under each type of environmental stimulus. Results The behavior experiment showed the difference among groups, both kidney deficiency group and spleen deficiency group acted out more apparent fear stress than normal group （without food stimulation P=0.039, with food stimulation P=0.037）. For example, under the environmental stimulus of light and no food, 3 groups leaned out for different frequency in 15 min： normal group showed（5.70± 1.636）times, spleen deficiency group showed（2.30±0.949）times and kidney deficiency group showed （0.05 ±0.224） times, which demonstrated fear stress and physical constitution are relevant; kidney deficiency group showed the most obvious fear stress among 3 groups, which proved the correlation between kidney deficiency and fear stress was more significant. Conclusion Kidney deficiency was a significant factor of fear stress, the correlation between them could be direct.