摘要
目的建立测定加味二妙丸中盐酸小檗碱含量的HPLC方法。方法采用Luna.C18柱(4.6mm×250mm,5μm),以乙腈:0.1%磷酸(45:55)为流动相,流速1.0ml/min,柱温30℃,检测波长245nm。结果盐酸小檗碱在0.071~1.42mg/ml浓度范围内有良好线性关系(r=0.9997),平均加样回收率为99.2%,RSD小于2.O%。结论本方法简便,快速,准确,重复性好,可用于该制剂的质量控制。
Objective To establish a HPLC method for the determination ofberberine hydroehloride in Jiawei Ermiao Wan. Methods The RP-HPLC system consisted of the Luna-C18 (4.6mm×250mm,5μm) column, the mobile phase consisted of acetonitrile-0.1%H3PO4(45: 55), the flow rate was 1.0mL ·min^-1, the column temperature was 30℃, The UV detector was set at 245nm. Results The linear response range was 0.071- 1.42 mg · ml^-1 (r=0.9998). The average recovery of mangiferin was 99.2%, and RSD〈2%. Conclusion The method was simple, rapid, accurate and repeatable. It could be applied in the determination of berberine hydrochloride in Jiawei Ermiao Wan.
出处
《国际中医中药杂志》
2013年第9期806-808,共3页
International Journal of Traditional Chinese Medicine