不同检验方法对慢性肝病的检验结果比较

Comparison test for chronic liver disease results from different test methods

目的:探讨不同检验方法对慢性肝病检验结果的影响。方法:采用酶联免疫吸附试验法(EUSA)、胶体金层析法(GIA)和聚合酶链式反应法(PCR)3种方法检验100例肝病患者血液指标,其中HBeAg、HBeAb均检查为阳性,对3种方法的检出率进行统计分析。结果:3种检测方法的检出率分别为80%、72%和93%,PCR定量检测的灵敏度明显高于ELISA法与GIA检测方法,差异具有统计学意义(P<0.05)。结论:PCR检测HBV—DNA其结果更精准,值得推广应用。

Objective： To study on effects of different testing method for chronic liver disease inspection result influence. Methods： Using enzymelinked immunosorbent assay （ELISA） methed,colloidal gold chromatography （GIA） and pelymerase chain reaction （PCR） method of three methods 1130 cases of patients with liver blood test indicators,including HBeAg,HBeAb were tested positive for inspection,the detection of the three methods for statistical analysis. Results： Three kinds of detection methods detection rate were 80% ,72% and 93% ,qtuantitative PCR detection sensitivity was obviously higher than that of the ELISA method and GIA detection methed,a statistically significant difference （P 〈 0. 05）. Conclusion： PCR detection HBV - DNA the result mere accurate,it might be worth for spreading application.