超细氯化钠粉的BALB/C小鼠骨髓微核实验

Micronucleus test of NaCl extrafine powder in BALB/c mice

目的:用骨髓微核试验观察超细氯化钠粉对BALB/C小鼠的毒性作用,为进一步研究矿盐粉的致突变性提供参考。方法:将50只小鼠随机分为5组,分别为阴性对照组,超细氯化钠粉低、中、高3个剂量组和阳性对照组(腹腔注射环磷酰胺),每组10只。高、中、低剂量组分别给予5.0、2.5、1.25 mg/kg剂量的超细氯化钠粉(pM2.5-10)连续灌胃14 d,观察试验期内小鼠的一般毒性症状、体质量增长情况和骨髓细胞微核率。结果:实验期间各组小鼠精神、外观均无异常。超细氯化钠粉高剂量组动物体质量在给药后第2～3天下降,中、低剂量组体质量未见异常。3种剂量氯化钠灌服组小鼠骨髓细胞微核率无明显变化(P>0.05)。结论:超细氯化钠粉对小鼠骨髓细胞无致突变作用。

Objective： Observing the toxic reaction and calculating the micronucleus rate of BALB/c mice administrated by different doses of NaCl extrafine powder to provide a reference for further mutagenesis studies.Methods： The experimental animals were randomly divided into five groups： negative control group,low-dose NaCl extrafine powder group,middle-dose group,high-dose group,and positive control group.Ten animals were in each group.By continous administration,the general toxicity,body weight growth,and bone marrow cell micronucleus in mice were observed during the experimental period.Results： No abnormal reactions in animal mind and appearance were observed.The growth of body weight in high-dose group decreased after 3rd day after administration,while the low-dose NaCl extrafine powder group and middle-dose group showed normal body weight.NaCl extrafine powder（pM2.5-10）within the dose of 5.0,2.5,1.25 mg/kg did not significantly affect the mouse bone marrow micronucleus rate.Conclusion： NaCl extrafine powder had no significant mutagenicity on mouse bone marrow cells.