不同时期和不同来源鼠胚玻璃化冷冻效果的比较

Effects of vitrifying mouse embryos from different periods and different sources

目的:比较不同时期、不同来源的鼠胚玻璃化冷冻的存活率以及发育为囊胚的成功率。方法:采用体外受精和体内受精的方式得到小鼠的2、4、8细胞期胚胎,对其进行玻璃化冷冻、解冻,观察胚胎的存活与囊胚形成,以未冷冻的胚胎作为对照组。结果:由体外受精和体内受精获得的2细胞期胚胎在冷冻复苏后的存活率分别为70.67%、72.06%,囊胚形成率分别为65.33%、67.62%;4细胞期胚胎冷冻复苏后的存活率分别为88%、89.71%,囊胚形成率分别为81.33%、83.82%;8细胞期胚胎冷冻复苏后的存活率分别为93.33%、94.12%,囊胚形成率分别为92%、94.12%。和对照组相比,2细胞和4细胞期胚胎冷冻复苏后囊胚形成率差异有统计学意义(P<0.05),而8细胞期的胚胎差异无统计学意义(P>0.05)。不同时期胚胎冷冻复苏后囊胚形成率间差异有统计学意义(P<0.05),受精方式对处于同一时期的胚胎在冷冻复苏后存活率和囊胚形成率均无明显影响。结论:玻璃化冷冻可以作为小鼠胚胎保存的有效方法,相对2、4细胞期胚胎,冷冻效果最好的是8细胞期胚胎。

Objective： To compare the post-thaw survival rate and the success rate in continuing to grow to blastocyst of vitrified mouse embryos from different periods and different sources.Methods： The 2-cell,4-cell,8-cell period mouse embryos were obtained through fertilization in vitro and in vivo respectively.These embryos were vitrified and thawed.We got the rate of their survival and blastocyst formation.And,we took those fresh embryos as the control group.Results： The survival rate of blastocyst formation of post-thaw 2-cell,4-cell and 8-cell period embryos from fertilization in vitro and in vivo were 70.67%/65.33% and 72.06%/67.62%,88.00%/81.33% and 89.71%/83.82% and 93.33%/92.00% and 94.12%/94.12% respectively.The blastocyst formation rate in the phrase of 2-cell and 4-cell frozen embryos was lower than the control group（P＆lt;0.05）.No significant difference was found between the group of 8-cell frozen embryos and the control group.In addition,fertilization modes had no significant effect on the survival rate and the blastocyst formation rate（P＆gt;0.05）.Conclusion： Vitrification technology is an effective method in preserving mouse embryos.And the 8-cell period embryos might have the best cryopreservation effect.