褪黑素受体MT1和转录因子Sp1在乳腺癌组织中的表达及临床意义

Expression and clinical significance of melatonin receptor MT1 and transcription factor Sp1 in breast cancer

目的:检测乳腺癌组织中褪黑素受体MT1与转录因子Sp1的表达,探讨两者表达的相关性及其临床意义。方法:采用免疫组织化学SP法和蛋白质印迹技术检测MT1和Sp1在90例乳腺癌组织和50例癌旁正常乳腺组织中的表达情况。结果:MT1在乳腺癌组织和癌旁正常乳腺组织中的阳性表达率分别为64.4%和22.0%,两者比较差异有统计学意义(P<0.01)。Sp1在乳腺癌组织和癌旁正常乳腺组织中的阳性表达率分别为74.4%和28.0%,两者比较差异有统计学意义(P<0.01)。蛋白质印迹半定量检测结果与免疫组织化学结果相一致。乳腺癌组织中MT1、Sp1的表达与TNM分期、肿瘤浸润及淋巴结转移呈正相关(P<0.05),与患者年龄、肿瘤大小、组织学分级无明显相关性(P>0.05)。乳腺癌组织中MT1的表达与Sp1的表达呈正相关(r=0.523,P<0.01)。结论:MT1、Sp1可能共同参与了乳腺癌的侵袭和转移,检测两者的表达对判断乳腺癌临床进展、预后推测具有一定的参考价值。

Objective： To explore the relationship and clinical significance between the expressions of melatonin receptor MT1 and transcription factor Sp1 in breast carcinoma.Methods： The expressions of MT1 and Sp1 in 90 specimens of breast cancer tissues and 50 specimens of adjacent normal breast tissues were detected by immunohistochemical streptavidin-peroxidase（SP） assay and western blotting.Results： There were significant differences in positive expression rates of MT1 and Sp1 between breast cancer tissues and adjacent normal breast tissues（64.4% vs 22.0%,P＆lt;0.01 for MT1,respectively,and 74.4% vs 28.0% P＆lt;0.01 for Sp1,respectively）.The results of western blotting were in accordance with the results of immunohistochemistry technique.The expressions of MT1 and Sp1 in breast cancer tissues were correlated with TNM stage,tumor invasion and lymphnode metastasis（P＆lt;0.05）,but not correlated with age of patients,tumor size and histological grade（P＆gt;0.05）.The MT1 expression positively correlated with Sp1 expression in breast cancer tissues（r= 0.523,P＆lt;0.01）.Conclusion： MT1 and Sp1 may be involved in the invasion and metastasis of breast cancer.The detection of both two expressions is valuable for judging clinical development and presuming prognosis.