银杏酚对SMMC-7721肝癌细胞和荷H22肝癌小鼠的抗癌作用

Inhibitory effect of ginkgols on SMMC-7721 liver cancer cells in vitro and liver cancer H22-bearing mice in vivo

目的:研究银杏酚对体外SMMC-7721肝癌细胞的毒性作用,及对体内荷H22肝癌小鼠肿瘤生长的抑制作用。方法:MTT法测定银杏酚对SMMC-7721细胞的抑制作用,计算半数抑制质量浓度IC50。建立小鼠肝癌H22实体瘤模型,并随机分为模型对照组,环磷酰胺组,银杏酚20、40、80、160 mg/kg组,分别于腹腔注射生理盐水,环磷酰胺,20、40、80、160 mg/kg银杏酚,连续给药7 d。于末次给药后24 h,眼球采血后脱颈处死小鼠,剥离瘤体,分别摘取脾脏、胸腺,计算抑瘤率、脾指数和胸腺指数,并作血常规检测。结果:银杏酚作用于SMMC-7721细胞24、48和72 h的半数抑制质量浓度IC50分别为(15.33±1.19)、(12.20±1.08)、(10.88±1.03)mg/L。银杏酚20、40、80、160 mg/kg组的抑瘤率分别为8.96%、39.81%、48.51%和28.36%,除外40 mg/kg组,其余组与环磷酰胺组(42.94%)比较,差异均有统计学意义(P均<0.05)。银杏酚组的胸腺指数较模型对照组均明显降低(P均<0.05);银杏酚组的外周血淋巴细胞数明显高于模型对照组与环磷酰胺组(P均<0.05)。结论:银杏酚于体外能抑制人肝癌SMMC-7721细胞生长,并呈时间和剂量依赖关系;于体内能显著抑制荷H22肝癌小鼠肿瘤生长,其抑瘤作用可能与调节机体的免疫功能有关。

Objective： To study the inhibitory effects of ginkgols on SMMC-7721 liver cancer cells in vitro,and on tumor growth of H22-bearing mice in vivo.Methods： The inhibitory effect of ginkgol on SMMC-7721 cells was evaluated by the survival rate of SMMC-7721 cells using MTT assay;then half maximal inhibitory concentration（IC50） was calculated.The solid tumor model of liver cancer H22-bearing mice was established,and all mice were randomly divided into model control group,cyclophosphamide（CTX） group,four ginkgol groups（20,40,80,160 mg/kg）,eight in each group.All mice in the groups received intraperitoneal injeciton of normal saline（0.2 mL each）,CTX（2 mg/kg body weight）,ginkgol 20,40,80,160 mg/kg,respectively,for 7-d.At 24 hours after the final injection,blood sample was collected by eye bleed;samples of tumor tissues,spleen and thymus were harvested after mice were killed.The inhibition rate of the anti-tumor,spleen index and thymus index were calculated.The levels of lymphocytes in peripheral blood were measured.Results： MTT assay showed that ginkgols inhibited the proliferation of SMMC-7721 cells with half maximal inhibitory concentration（IC50） values of（15.33±1.19）,（12.20±1.08） and（10.88±1.03） mg/L for 24,48,and 72 hours,respectively.The inhibition rate of 20,40,80 and 160 mg/kg group were 8.96%、39.81%、48.51% and 28.36%,respectively.Except for 40 mg/kg group,there were significant differences in inhibition rate between the other ginkgols groups and model control group（both P＆lt;0.05）.The levels of lymphocytes in ginkgols groups from peripheral blood were significantly increased,compared with CTX group（P＆lt;0.01）.Conclusion： Ginkgols showed markedly inhibitory effects on cell proliferation of human hepatic carcinoma cell lines SMMC-7721 in vitro,and on tumor growth of H22-bearing mice,which could be associated with the regulation of immune function.