Visfatin基因过表达对PCOS模型大鼠血浆胆固醇代谢和肝胆固醇合成的影响

Effects of Visfatin gene Overexpression on Hepatic Cholesterol Metabolism and Expression of SREBP-2 Gene in Rats with Polycystic Ovarian Syndrome

目的探讨内脂素(visfatin)对PCOS模型大鼠血浆胆固醇代谢和肝胆固醇合成的影响。方法采用SD清洁雌性大鼠注射脱氢表雄酮(DHEA)和胰岛素诱导建立PCOS模型(n=20),并以等量油剂代替DHEA为对照组(n=20),大鼠visfatin基因真核表达质粒和空质粒分别转染PCOS组和对照组,ELISA方法测定血浆visfatin水平;RT-PCR测定肝HMG-CoA还原酶和SREBP-2 mRNA表达的变化。结果 visfatin质粒注射72 h后较注射前:(1)血浆visfatin水平升高(P<0.05);(2)HMG-CoA还原酶mRNA及SREBP2 mRNA的表达较转染前均升高(P<0.05),正常大鼠visfatin基因真核表达质粒注射组HMG-CoA还原酶mRNA的表达较转染前升高(P<0.05)。结论 visfa-tin过表达可能在PCOS模型大鼠脂代谢中起调节作用。

Objective Objective To study the effects of visfatin gene overexpression on hepatic cholesterol metabolism and expression of SREBP-2 gene in rats with Polycystic Ovarian Syn- drome（pcos）.Methods Rat model induced by subcutaneous injecting dehydroeplandrosterone （DHEA） and Insulin on the 42-day-old female SD rats were injected daily with DHEA for up to 28 days and rats in group 2 injected with oil at the same time. The control plasmid pcDNA3.1 （＋） and recombinant plasmid pcDNA3.1-visfatin were transfected into rats in control group and Polycystic Ovarian Syndrome group. Serum level of visfatin in rats of both groups were deter- mined with ELISA method;The plasma cholesterol, hepatic HMG-CoA reductase and sterol regulatory element binding protein 2（SREBP-2） mRNA were evaluated by RT-PCR before and after transfection. Results The expression of the serum level of visfatin was significantly in- creased in after 3 d of pcDNA3.1-visfatin transfection than those in the control plasmid pcD- NA3.1 （＋） rats（P〈0. 05）;HMG-CoA reductase mRNA level and SREBP-2 level of group re- combinant plasmid pcDNA3.1-visfatin was significantly increased than that those in the control plasmid pcDNA3.1 （＋） rats （P〈O. 05）and HMG-CoA reductase mRNA level of group NT was significantly increased than that those in the control plasmid pcDNA3.1 （＋） rats （P〈O. 05）.Con- clusion The transfection of visfatin plasmid was related on blood I ipid level in Rats with PCOS.