摘要
建立了一种三重串联四级杆液质联用法(LC/QQQ)测定动物源性食品中喹烯酮残留量的方法。动物源性食品样品经匀浆、超声提取后经固相萃取柱(中性氧化铝AlumiaN(1g,3mL))净化,采用LC/QQQ电喷,正离子模式,多反应监测(MRM)。喹烯酮在0.02-0.2mg/kg范围内标准曲线的相关系数大于0.999。在不同添加水平下的回收率在83.5-107.8%之间,定量限(LOD)为20μg/kg,检出限(LOD)为10μg/kg.
A LC/QQQ method was established for determination of quinotone in foodstuffs of animal origin. The sample was extracted by a homogenater and an ultrasonic cleaner, purified by a solid phase extraction （SPE） cartridges （Alumia N（1g,3mL） ） and detected by the positive electrospray ionization （ESI＋） method under multiple reaction monitoring （MRM） mode. The calibration curves were linear （r2=0.999） in the concentration range of 20 - 200μg/kg. The extraction recoveries ranged from 83.5% to 107.8% in different spiked concentrations samples. The LOQ and LOD were 20μg/kg and 10 μg/kg.
出处
《安徽化工》
CAS
2013年第4期90-92,共3页
Anhui Chemical Industry
基金
安徽省科技攻关计划项目(08010304219)
