碳源和氮源对异养硝化好氧反硝化菌株Y1脱氮性能的影响

Effect of carbon and nitrogen sources on nitrogen removal by a heterotrophic nitrification-aerobic denitrification strain Y1

从焦化废水活性污泥中筛选到一株高效脱氮细菌,命名为Acinetobacter sp.Y1.本实验对菌株Y1在不同碳源、氮源、碳氮比及底物浓度下的脱氮特性进行了研究,结果表明,菌株Y1可以利用氨氮、亚硝氮和硝氮生长,不能利用羟胺;以氨氮为唯一氮源进行硝化作用时,柠檬酸钠和乙酸钠是最佳碳源,最佳碳氮比为15,菌株Y1可降解高浓度氨氮,在36h内将400mg·L-1氨氮全部去除,1600mg·L-1氨氮的去除率可达21.3%,最大降解速率随着初始氨氮浓度的升高而增大.以硝氮或亚硝氮为唯一氮源进行反硝化时,菌株Y1可以适应高浓度氮源但不能完全去除氮源,当碳氮比为20,经36h培养硝氮和亚硝氮的去除率均达到100%.

Acinetobacter sp. Y1, a high effective heterotrophic nitrification bacterium, was isolated from the activated sludge of a coking wastewater treatment facility. The characteristics of nitrogen removal by strain Y1 at different carbon sources, C/N ratios, nitrogen sources and substrate concentrations were investigated. The result shows that Acinetobacter sp. Y1 can remove different forms of nitrogen sources. The most efficient ammonium removal and growth rate for Y1 was occurred at C/N=15 when sodium citrate was supplemented as the carbon source, ammonium removal rate reached 99% and OD600max was 1.432. High concentration of ammonium ranging from 400 mg · L-1 to 1600 mg · L-1 could be degraded well by strain Y1. The most efficient nitrogen removal was occurred at C/N=20 when nitrite or nitrate was treated as a sole nitrogen source.