新农1号狗牙根愈伤组织诱导及再生研究

Callus Induction and Regeneration System of Cynodon dactylon(L.) Pers.'Xinnong No.1'

以新农1号狗牙根(Cynodon dactylon(L.)Pers.‘Xinnong No.1’)成熟颖果为外植体,研究不同激素浓度组合对愈伤组织诱导、继代及分化的影响。结果表明:在愈伤组织诱导中,适宜的培养基为添加2.00mg.L-1 2,4-D+0.01mg.L-1 6-BA+500mg.L-1脯氨酸的MS培养基,出愈率可达71.2%,愈伤形态为半透明水渍状且淡黄色颗粒较多;继代改造培养基中添加2,4-D 0.5mg.L-1+6-BA 0.5mg.L-1+ABA 2.0mg.L-1+GSH 2.0mg.L-1的MS培养基上培养4周后,愈伤组织增殖率可达41.3%;分化过程中,在不添加任何激素的1/2MS培养基中培养10d后,将胚性愈伤组织转入添加6-BA 1.0mg.L-1的MS培养基中,很快绿点变密,继而分化成苗,愈伤组织再生频率达25%,小植株形成的强度为16.7%。

Influences of different hormone concentrations on callus induction were studied using seeds of Cynodon dactylon （L.） Pers. ＇Xinnong No. 1＇ as explants. The effects of different subculture media on callus proliferation and differentiation were investigated. The main results were： for callus induction, MS medium added with 2.00mg＂ L-12,4-Dq- 0.01 mg- L 16_BAq_ 500mg. L lprolinewassuitable; and the induction rate could reach 71.2%. The callus was translucent, water-soaked form with light yellow particles. The regeneration medium added with 2,4-D 0.5 mg ~ L-1 q- 6-BA 0.5 mg ~ L-~ q- ABA 2.0 mg~ L 1 q-GSH 2.0 mg ~ L-1 was beneficial to embryogenic callus growth and callus proliferation rate reached 41.3%. Transferring embryogenic callus into 1/2MS medium without any hormones for 10 days, then transferring into MS medium added with 6-BA 1.0 mg ~ L-1 , soon green dot of embryogenic callus became dense and then differentiated seedlings. The callus regeneration frequency was 25 ~ and the plantlets rate was 16.7%.