ARHI基因在乳腺癌组织中的表达及其意义

Expression of ARHI gene in breast cancer tissue and its significance

目的:探讨ARHI基因在乳腺癌组织中的表达及意义。方法:收集临床80例乳腺癌患者癌组织、40例癌旁乳腺组织和20例正常乳腺组织,采用免疫组织化学法和RT-PCR法检测ARHI基因在乳腺癌组织、癌旁乳腺组织及正常组织中的表达。结果:与正常组织和癌旁组织相比较,ARHI蛋白和ARHImRNA水平在乳腺癌组织中的表达均明显降低(P<0.01),并且两者随组织病理分级的升高而降低(P<0.05),有淋巴结转移的低于无淋巴结转移者(P<0.05),但在不同组织学类型中的表达差异无统计学意义(P>0.05)。结论:ARHI基因表达的下调,说明ARHI在乳腺癌的发生、发展中具有重要作用,并为乳腺癌的诊断和预后提供依据。

Objective： To explore the expression and significance of ARHI gene in breast cancer tissue. Methods： Eighty breast cancer tissue samples, forty breast tissue samples adjacent to breast cancer, and twenty normal breast tissue samples were collected, immu- nohistochemical method and RT - PCR were used to detect the expression levels of ARHI gene in breast cancer tissue, breast tissue adjacent to breast cancer, and normal breast tissue. Results： The expression levels of ARHI protein and ARHI mRNA in breast cancer tissue were statistically significantly lower than those in normal breast tissue and breast tissue adjacent to breast cancer （ P 〈 0. 01 ）, which decreased with the increase of histopathological grades （ P 〈 0. 05 ） ; the expression levels of ARHI protein and ARHI mRNA in breast cancer tissue with lymph node metastasis were statistically significantly lower than those in breast cancer tissue without lymph node metastasis （ P 〈 0. 05 ）, but there was no statistically significant difference among breast cancer tissue of different histological types （ P 〉 0.05 ） . Conclusion： The down- regulation of ARHI gene expression shows that ARHI plays an important role in occurrence and development of breast cancer, which provides a basis for diagnosis and prognosis of breast cancer.