四川白鹅MTP基因的组织表达特性研究

Tissue Expression Characteristics of MTP Gene in Sichuan White Geese

本研究采用RT—PcR方法以四川白鹅（Ansercygnoides）为研究材料，探讨了1、10、13、14和16周龄肝脏、小肠、脑、胸肌、腹肌、腹脂、皮脂和心脏等组织中微粒体甘油三酯转运蛋白（MTP）基因的表达特性。结果显示：1周龄，MTP基因只在小肠组织中表达；10周龄，除在肝脏、小肠组织中，首次发现脑组织为M即基因特有表达组织；MTP基因在肝脏和小肠中的表达量随周龄增加呈逐步上升趋势；1-13周龄，小肠〉肝脏（P〈0．05）；14～16周龄，小肠〈肝脏（P〉0．05）；填饲期，肝脏组织中MTP表达无显著变化（P〉0．05），而小肠组织肘即表达水平却显著升高（P〈0．05）。

With Sichuan white geese as experimental materials firstly,RT-PCR method was adopted to study the expression characteristics of microsmal triglyeeride transfer protein （MTP）gene in liver, small intestine, brain, breast muscle, abdominal muscle, abdominal fat and heart tissue at 5 stages of growth development （1,10,13,14 and 16 weeks of age） was studied. The results indicated the expression of MTP gene was detected only in small intestine at 1 weeks of age;except in the liver and intestine tissues,MTP gene was firstly discovered in the brain tissues at 10 weeks of age;the expression level of MTP gene in liver and intestine increased with aging （P〈0.05）;the expression of MTP gene in intestine was higher than which in liver from 1 to 13 weeks of age,then which was lower than that in liver from 14 to 16 weeks of age reversely;during the overfeeding, the level of MTP mRNA had no significant change in liver tissues （P〉0.05）, but increased significantly in small intestine tissues （P〈0.05）. Therefore,MTP gene was a very important factor in the regulation and control of fat deposition and transfer in Sichuan white goose.