柴黄颗粒对人工感染鸡传染性支气管炎的防治研究

Prevention and Treatment of Chaihuang Granule on Chickens Infected Artificially with Avian Infectious Bronchitis

为探讨柴黄颗粒对鸡传染性支气管炎的防治效果,将400只15日龄海兰褐鸡随机均分8组:柴黄颗粒预防组,低、中、高剂量治疗组,中、西药对照组,模型对照组和健康对照组。除健康对照组外,其余各组均接种鸡传染性支气管炎病毒M41,预防组于攻毒前7 d饮水中加入柴黄颗粒进行预防,各药物治疗组于攻毒48 h后进行药物治疗,直到临床症状消失。结果表明,感染前7 d给予2 g/L柴黄颗粒对人工感染鸡传染性支气管炎有较好的保护作用,使其发病率降低至50%,与模型对照组比较差异显著(P<0.05);4 g/L柴黄颗粒治疗组死亡率为5%,与模型对照组比较差异极显著(P<0.01),治愈率为92%,与西药对照组差异极显著(P<0.01),效果优于中药对照组。柴黄颗粒中剂量组攻毒第7天抗体滴度达到1 531.33±389.91,与模型对照组差异极显著(P<0.01),并随时间增加而增加。2 g/L预防剂量和4 g/L治疗剂量的柴黄颗粒对鸡呼吸型传染性支气管炎有较好防治效果。

In order to probe into the prevention and treatment effect of Chaihuang granule （CG）on avian infectious bronchitis,four hundred 15 days of age Hy-Line brown chicken were randomly divided into 8 groups：CG prevention group, treatment groups with CG at low, medium and high dosage, Chinese and western medicine control group, model and healthy control group. The chicken were challenged with avian infectious bronchitis virus （IBV） M41 except healthy control group. Before 7 days of challenge, CG was added into drinking water in prevention group. After 48 hours, chickens in treatment groups were treated with drugs respectively until the clinical symptoms had disappeared. The results showed that the morbidity of prevention group （2 g/L） was reduced up to 50%, which was significantlylower than model control group （P〈0.05）. The mortality of CG treatment group at dosage of 4 g/L was 5% , and extremely significantly lower than that of model control group （P〈0.01）;hs cure rate （92%）was extremely significantly higher than that of western medicine control group （P〈0.01）,and the eurative effect was better than Chinese medicine control group. In treatment group with CG at dosage of 4 g/L on day 7 after challenged, antibody titer reached 1 531.33±389.91, was extremely significantly higher than that of model control group （P〈0.01）, and increased along with time. These results indicated that CG at prophylactic dosage （2g/L）and therapeutic dosage （4g/L） had better prevention and treatment effeet on chickens infected artificially with avian respiratory infectious bronchitis.