小菜蛾p38 MAPK基因的克隆与生物信息学分析

Cloning and Bioinformatic Analysis of p38 MAPK Gene from Plutella xylostella

小菜蛾（PhttellaxylostellaL．）是一种危害十字花科植物的世界性害虫，研究其基因功能为寻找新的小菜蛾防治方法具有重要意义。本研究克隆了小菜蛾p38MAPK基因的开放阅读框（ORF），并根据其DNA序列推导出了蛋白一级结构，发现该基因编码一个含有349个氨基酸残基的蛋白。通过SMART网站分析发现该蛋白在第20～304位氨基酸残基区域存在着一个典型的丝氨酸／苏氨酸蛋白激酶结构域，说明它是一个潜在的丝氨酸／苏氨酸蛋白激酶。Blast比对发现Pxp38基因与家蚕Bmp38基因、酿酒酵母ScHOGl基因、人类Homosapiensp38基因在蛋白～级结构上的相似性分别是91．7％、51．6％和78．6％，说明p3sMAPK基因在进化上具有较高的保守性。·

Plutella xylosteUa L. is a major agricultural pest around the world, so the research on its gene function is important and meaningful to its biological control. In this study, the open reading frame （ORF） of Plutella xylostella p38 MAPK gene was cloned, and its protein primary structure was deduced according to the DNA sequence. It was found that the Pxp38 MAPK encoded a protein with 349 amino acid residues. By sub- mitted to the SMART website, a classic Ser/Thr protein kinase domain was found between 20 -304 amino acid residues, which indicated that Pxp38 MAPK gene was a putative Ser/Thr protein kinase. The similarity be- tween Pxp38 and Bmp38, ScHogl, Homo sapiens p38 was 91.7%, 51.6% and 78.6% respectively, which proved that p38 MAPK was highly conserved in evolution.