黄芪酵母菌发酵液抗肿瘤活性研究

Study on Antitumor Activity of Radix Astragali Budding Fungus Fermentation

目的:观察不同剂量的黄芪酵母菌发酵液对体外培养H22细胞增殖及细胞凋亡和细胞周期的影响。方法:采用MTT法测定不同浓度黄芪发酵液作用时间不同(24 h、48 h、72 h)对H22细胞的生长抑制作用;采用流式细胞仪PI染色法检测黄芪发酵液对H22细胞周期分布的影响;采用流式细胞仪Annexin V-FITC/PI双标法检测对H22细胞凋亡率的影响。结果:随着黄芪发酵液浓度增大和时间延长,对H22肿瘤细胞的抑制作用也更加明显,72 h时最高抑制率为32.54%;随着黄芪发酵液浓度的增加,G0/G1细胞的比率逐渐增加,高浓度组细胞凋亡率为(15.25±6.07)%。结论:黄芪酵母菌发酵液对H22细胞生长有一定抑制作用,诱导肿瘤细胞发生G1期阻滞,并使细胞凋亡。

Objective： To investigate the effect of different dosage of radix astragali budding fungus fermentation broth on H22 cell proliferation, apoptosis and cell cycle. Methods： MTY assay was applied to evaluate the growth inhibition of H22 caused by fermentation broth in different density and in different time （24 h, 48 h and 72 h）, PI staining method with flow cytometry to determine H22 cell cycle distribution, and Annexin V/PI bi-staining method to determine H22 apoptosis rate. Results： With the increasing concentration and prolonged fermentation, the inhibition effect became more significant, and the highest inhibition rate was 32. 54% in 72 h. The ratio of G0/G1 cell gradually increased with the concentration of the Radix Astragali fermentation increasing, and the apoptosis rate in high-dose group was （ 15.25 ± 6.07 ） %. Conclusion ： Radix Astragali budding fungus fermentation broth has a certain inhibition effect on H22 cell growth. It can induce the G1 arrest and allow the cells apoptosis.