摘要
目的:研究茶多酚对大鼠离体心肌缺血再灌注损伤的作用。方法:将32只健康雄性SD大鼠随机分为四组:假手术组(SHAM组)、缺血再灌注组(I/R组)、缺血后处理组(IPO)、茶多酚后处理组(TP组)。观察茶多酚对缺血再灌注后血液中肌酸激酶(CK)和过氧化物歧化酶(SOD)含量的影响。ELISA法测定HSP70含量。结果:I/R组、IPO组和TP组的CK含量明显高于SHAM组(P<0.05),同时SHAM组、IPO组和TP组的SOD含量明显高于I/R组(P<0.05),表明I/R组的心肌损伤相对严重。四组间HSP70含量的差异均有统计学意义。结论:茶多酚可能通过HSP70对大鼠心肌缺血再灌注损伤产生保护作用。
Objective:To observe the effect of Tea polyphenols on myocardial ischemia-reperfusion injury of rat hearts.Menthods:32 male Sprague-Dawley rats were randomly divided into four groups:sham-operation group(SHAM group),ischemia-reperfusion group(I/R group),ischemic postconditioning group(IPO group) and tea polyphenols postconditioning group(TP group).Then to analysis the impact of Tea polyphenols on CK and SOD after myocardial ischemia reperfusion injury of rats.Results:Content of CK was higher in I/R group,IPO group and TP group than that in SHAM group(P0.05),moreover content of SOD was higher in SHAM group,IPO group and TP group than that in I/R group(P0.05).This showed that the myocardial damage was comparatively serious in I/R group.There was a significant difference of content of HSP70 among four groups(P0.05).Conclusion:Tea polyphenols may have protected impact to myocardial ischemia-reperfusion injury of rats through HSP70.
出处
《长治医学院学报》
2013年第4期257-259,共3页
Journal of Changzhi Medical College