氧化应激与非肥胖多囊卵巢综合征关系

Relationship between oxidative stress and polycystic ovary syndrome in non-obese women

目的探讨氧化应激与非肥胖多囊卵巢综合征(PCOS)的关系。方法问卷调查湖北省武汉地区42例非肥胖PCOS患者和46名对照妇女的基本特征,检测其血清丙二醛(MDA)和8-羟基脱氧鸟苷(8-OHdG)等水平,并对MDA和8-OHdG与其他指标的相关性进行Spearman相关分析。结果非肥胖PCOS组患者血清中胆固醇(CHO)、总睾酮(TESTO)、促黄体激素(LH)、空腹胰岛素(FI)、胰岛素抵抗指数(HOMA-IR)、MDA和8-OHdG水平均高于对照组(均P<0.05);卵泡刺激素(FSH)水平低于对照组(Z=-2.942,P<0.01);相关分析结果显示,MDA和8-OHdG水平均与HOMA-IR(r=0.434,P<0.01;r=0.304,P<0.01)、TESTO(r=0.339,P<0.01;r=0.378,P<0.01)、LH(r=0.217,P=0.023;r=0.326,P<0.01)呈正相关,提示血清MDA和8-OHdG水平与PCOS的高胰岛素抵抗、高雄血症以及卵巢功能紊乱有关。结论非肥胖PCOS患者血清氧化应激水平高于健康对照组,且这种高氧化应激状态与非肥胖PCOS患者的高胰岛素抵抗和高雄血症密切相关。

Objective To investigate the relationship between oxidative stress and polycystic ovary syndrome （PCOS） in non-obese women. Methods A case-control study involving 42 newly diagnosed non-obese PCOS patients and 46 nomal female volunteers was performed. Information on general characteristics of the subjects were collected with a questionnaire survey and serum malondialdehyde （MDA）and 8-hydroxy-desoxyguanosine （8-OHdG）levels were detected. Correlations of serum MDA and 8-OHdG levels with other variables were analyzed using Spearman correlation analysis. Results Compared to non-PCOS controls,the non-obese PCOS patients had significantly higher concentrations of cholesterol （ CHO）, total testosterone （ TESTO）, luteinizing hormone （ LH）, fasting insulin （ FI）, homeostasis model as- sessment of insulin resistance （HOMA-IR）, MDA, and 8-OHdG （P 〈 0. 05 for all）. Whereas follicle stimulating hormone （FSH） level was significantly lower in PCOS patients than in the controls （P 〈 0. 01 ）. In the PCOS group, MDA and 8-OHdG were significantly associated with HOMA-IR （ r = 0. 434, P 〈 0.01 ; r = 0. 304, P 〈 0. 01, respectively ）, TESTO （ r = 0. 339, P 〈 0. 01 ; r = 0. 378, P 〈 0.01, respectively）, and LH （ r = 0. 217, P = 0. 023 ; r = 0. 326, P 〈 0. 01 ）, indicating that serum MDA and 8-OHdG had associations with hyperinsulinemia, hyperandrogenism, and ovarian dysfunction. Con- clusion Serum oxidative stress of non-obese PCOS patients is significantly higher than that of healthy control group. The mechanism of the high oxidative stress may be closely associated with hyperinsulinemia and hyperandrogenism in non- obese PCOS patients.