非酒精性脂肪肝小鼠肝组织PPARα和UCP-2表达

Expressions of PPARα and UCP-2 mRNA in hepatic tissue in NAFLD mice

目的探讨过氧化物酶增殖活化受体α(PPARα)和解偶联蛋白2(UCP-2)在非酒精性脂肪肝病(NAFLD)小鼠肝组织中的表达。方法雄性成年ICR小鼠30只随机分成3组,每组10只,即对照组(普通饲料)、模型A组(普通饲料4周+高脂饲料4周)、模型B组(高脂饲料8周),分别测定肝脏指数并制作肝脏病理切片,采用反转录-聚合酶链反应(RT-PCR)方法,测定小鼠肝组织中PPARα和UCP-2 mRNA表达。结果对照组、模型A、B组小鼠肝指数分别为(3.89±0.87)%、(7.42±0.95)%、(9.38±1.07)%,模型组小鼠肝指数均高于对照组(P<0.01),模型小鼠肝脏脂肪变性明显;模型A、B组小鼠肝组织中PPARαmRNA表达量分别为(0.63±0.33)、(0.45±0.19),低于对照组的(1.16±0.27)(P<0.01),模型A、B组小鼠肝组织中UCP-2 mRNA表达量分别为(0.67±0.76)、(0.89±0.52),高于对照组的(0.25±0.13)(P<0.01)。结论发生NAFLD的小鼠肝组织中PPARα和UCP-2 mRNA表达异常。

Objective To explore the expressions of peroxisome proliferator-activated receptor α （PPARα） and un- coupling proteins 2 （ UCP-2 ） in hepatic tissue in the mice with non-alcohlic fatty liver disease （ NAFLD ）. Methods Thirty mature male ICR mice were randomly divided into three groups （ 10 in each group）. The mice in control group were fed with normal diet; the mice in experimental group A were fed with normal diet for 4 weeks; and then with high- fat diet for 4 weeks; and the mice in experimental group B were fed with high-fat diet for 8 weeks. Liver index were measured. The expressions of PPARa and UCP-2 mRNA in hepatic tissue were examined with reverse transcription poly- merase chain reaction（ RT-PCR）. Results The liver index for the mice in control group, experimental group A and ex- perimental group B were 3. 89 ±0. 87,7. 42 ±0. 95 ,and 9. 38 ±1.07 ,respectively. In the mice of experimental group,the liver index was significantly higher than that of the control mice（ P 〈 0.01 ）. The steatosis of hepatic tissue in experimen- tal group was obvious. PPARa mRNA expressions in hepatic tissue of mice of experimental group A（0. 63 ±0. 33 ）and experimental group B （ 0.45 ±0. 19 ） were dramatically decreased compared to normal group （ 1.16 ± 0. 27 ） （ P 〈 0. 01 ）. UCP-2 mRNA expressions in hepatic tissue of mice of experimental group A （ 0. 67 ± 0. 76 ） and experimental group B （ 0. 89 ± 0. 52 ） were dramatically increased compared to normal group （ 0. 25 ± 0. 13 ） （ P 〈 0. 01 ）. Conclusion The ex- pression of PPAR a and UCP-2 in hepatic tissue of NAFLD mice are abnormal.