蛙皮素在胃癌及其癌前疾病和病变中表达变化

Change of bombesin expression in gastric cancer and premalignant lesion

目的了解蛙皮素在胃癌及癌前疾病(病变)中表达变化,探讨蛙皮素作为胃癌诊断生物学标志物的价值。方法采用免疫组化法和逆转录-聚合酶链反应(RT-PCR)法分别检测蛙皮素蛋白和mRNA在各组胃黏膜组织中的表达。结果免疫组化显示,蛙皮素表达水平在对照组(0.125 9±0.024 8)、浅表性胃炎组(0.131 8±0.037 1)、萎缩性胃炎组(0.111 4±0.019 4)较低;不典型增生组(0.338 7±0.046 5)和胃癌组(0.394 6±0.062 1)均明显高于前3组(P<0.05);胃癌组高于不典型增生组。RT-PCR结果显示,对照组(0.105 6±0.029 7)、浅表性胃炎组(0.110 0±0.037 1)、萎缩性胃炎组(0.091 4±0.021 3)的蛙皮素mRNA表达水平较低;不典型增生(0.301 7±0.062 5)与前3组相比,明显升高(P<0.05);胃癌组(0.695 8±0.072 4)明显高于前4组(P<0.05)。结论蛙皮素在胃癌的演变过程中起促进作用,且对于病变的不同阶段具有一定的提示及监控作用。

Objective To investigate the value of bombesin （BN） as a biological marker of gastric cancer by stud- ying the expression of BN in gastric cancer and premalignant lesion. Methods Immunohistochemical and reverse tran- scription PCR（RT-PCR） were used to determine the expression and distribution of protein and mRNA of BN in gastric cancer cases and the controls. Results Compared with that of the control（0. 125 9 ±0. 0248） ,the patients with chronic superficial gastritis （ 0. 1318 ± 0. 0371 ）, and the patients with chronic atrophic gastritis （ 0. 111 4 ± 0. 019 4 ） the BN ex- pressions in patients with atypical hyperplasia（0. 338 7 ±0. 046 5 ） and gastric cancer（0. 394 6 ± 0. 062 l ）were significantly higher（ P 〈 0. 05 for all）and the BN expression in gastric cancer patients was higher than in atypical hyperplasia patients. The expression of BN mRNA in the control （ 0. 105 6 ±0. 029 7 ）, chronic superficial gastritis （ 0. 110 0 ± 0. 037 1 ） and chronic atrophic gastritis（0.091 4 ± 0.021 3 ） were lower than that of gastric mucosal atypical hyperplasia （ 0. 301 7 ± 0. 062 5 ） （ P 〈 0. 05 ） and the BN mRNA expression in gastric cancer patients （ 0. 695 8 ± 0. 072 4 ） was higher than that of atypical hyperplasia patients （ P 〈 0. 05 ）, with significant differences between gastric cancer patients and cont- ols,chronic superficial gastritis patients and chronic atrophic gastritis patients. Conclusion BN plays a catalytic role in the process of gastric carcinogenesis, which could be a predictor for different stages of gastric disease and carcinosenesis.