宁夏社区居民代谢综合征NF-κB及TNF-α水平分析

Expression levels of NF-κB and TNF-α in community residents with metabolic syndrome in Ningxia

目的探讨宁夏社区居民代谢综合征(MS)患者与健康者核因子-κB(NF-κB)、肿瘤坏死因子(TNF-α)表达水平的差异,为宁夏地区人群防治MS提供理论基础。方法选取MS患者及健康对照组各180例,采用ELISA双抗体夹心法测定其血清NF-κB、TNF-α表达水平,分析2组差异,MS危险因素分析采用多因素logistic回归分析。结果病例组NF-κB表达水平为(1.99±1.42)ng/mL,对照组为(1.85±2.0)ng/mL,病例组高于对照组,差异有统计学意义(t=-3.15,P<0.05);病例组TNF-α表达水平为(41.04±25.11)g/mL,对照组为(30.5±18.11)g/mL,病例组高于对照组,差异有统计学意义(P<0.05);多因素logistic回归分析显示,吸烟(OR=1.97,95%CI=1.07～3.63)、NF-κB(OR=1.85,95%CI=1.03～3.33)、TNF-α(OR=1.02,95%CI=1.01～1.03)是MS的危险因素。结论 MS患者血清NF-κB、TNF-α表达水平升高,吸烟、NF-κB、TNF-α是MS的危险因素。

Objective To investigate expression levels of nuclear factor kappa B （NF-κB） and tumor necrosis factor alpha（TNF-α） in community residents with metabolic syndrome（MS）and healthy controls in Ningxia Hui Autonomous Region and to provide theoretical basis for prevention and control of metabolic syndrome. Methods Totally 180 MS patients and 180 healthy controls were selected for a case-control study. The expression levels of NF-KB and TNF-α were measured with double antibody sandwich enzyme-linked immunosorbent assay（ELISA）. Paired t test was used to analyze the differences in expressions of NF-κB and TNF-α between the two groups and logistic regression was used to analyze relevant risk factors. Results The expression level of NF-KB in the patients was 1.99 ±1.42 ng/mL and 1.85±2.0 ng/mL in the controls. The NF-KB level in the patients was significantly higher than in the controls （ t = - 3. 15, P 〈 0.05 ）. The expression level of TNF-ct in the patients was 41.04 ±25.11 g/mL and 30. 5 ±18. 11 g/mL in the controls. The TNF-α level in the patients was significantly higher than in the controls（ t = - 4. 85, P 〈 0. 01 ）. Lo- gistic regression analysis suggested that smoking （ odds ratio [ OR ] = 1.97 ; 95 % confidence interval [ 95 % C11 = 1.07 - 3.63） ,NF-KB expression（ OR = 1.85;95% CI = 1.03 - 3.33 ）, and TNF-αexpression （ OR = 1.02;95% CI = 1.01 - 1.03 ） could significantly increase the risk of MS. Conclusion The NF-KB and TNF-α are increased in metabolic syn- drome patients. Smoking,NF-κB and TNF-α expressions are risk factors for MS.