阴虚、阳虚体质血清对肺癌细胞NF-κB、P16基因表达的影响

Effects of yin-yang deficiency constitutional types serum on NF-κB and P16 expression in lung cancer cell

目的:探讨阴虚、阳虚体质血清对肺癌细胞生长的部分机制。方法:肺癌细胞A549培养使用10%的人血清,采用MTT分析细胞增殖,流式细胞仪分析细胞周期,用TR-PCR及Western Blot的方法测定基因表达。结果:阴虚、阳虚体质血清培养的细胞增殖数明显高于平和质(P<0.05),G_0/G_1期的细胞数低于平和质(P<0.05),而S期的细胞数明显高于平和质(P<0.05),而且阴虚、阳虚体质血清培养的细胞内表达的核转录因子-κB(NF-κB)高于平和质(P<0.05,P<0.01),P16则低于平和质(P<0.05)。结论:阴虚、阳虚体质血清能促肺癌细胞增殖,可能的机制是促进细胞内NF-κB基因表达,而抑制P16基因表达有关。

Objective： To investigate mechanism of yin-yang deficiency constitution types serum on lung cancer cell growth. Methods： A549 cell culture technique was treated with 10% human serum. Cell proliferation was observed by MTT metabolism measuring. Flow cytometry was performed to track cell cycle progression. Western bolts and RT-PCR were performed to evaluate gene expression. Results： The number of cell proliferation of yin-yang deficiency constitutional types serum was significantly higher than that of gentleness type senum （P〈0.05）. Flow cytometry （FCM） analysis indicated that the GJG~ phase fraction ratio of yin deficiency, yang deficiency serum group was lower than that of serum of gentleness type （P〈0.05）, while the S-phase fraction ratio was higher than that of serum of gentleness type （P〈0.05）. Western blots and RT-PCR results displayed that serum of constitution of yin deficiency, yang-deficiency elevated the expression of NF-~cB （P〈0.05, P〈0.01） and decreased the expression of P16 than serum of gentleness type did （P〈0.05）. Conclusion： These results suggest that serum of constitution of yin deficiency, yang deficiency significantly sitimulate cell proliferation. This effects may be related to increasing the NF-~cB expression and decreasing P16 expression.