次乌头碱与甘草苷、甘草次酸配伍的减毒作用

Detoxicated effect of compatibility of hypaconitine and liquiritin,glycyrrhetinic acid

目的:探讨次乌头碱(HA)与甘草苷、甘草次酸配伍对心肌细胞的减毒作用。方法:以新生SD乳鼠心肌细胞为研究对象,观察甘草苷、甘草次酸对HA引起心肌细胞的形态变化和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性的影响。结果:与120μmol/L HA组比较,120μmol/L甘草次酸、480μmol/L甘草苷与120μmol/L HA配伍可使SOD、GSH-PX的活性由(56.38±0.41)U/mL和(81.89±3.67)U/mgprot分别增加到(65.39±0.25)U/mL、(101.69±7.17)U/mgprot和(62.69±0.54)U/mL、(100.85±7.32)U/mgprot(P<0.05),120μmol/L的甘草次酸和120μmol/L HA配伍作用更显著(P<0.01)。结论:480μmol/L甘草苷、120μmol/L甘草次酸与120μmol/L HA配伍可以降低HA对心肌细胞的毒性作用,增强其活力,这与甘草苷、甘草次酸减少了HA对心肌细胞的氧化损伤有关。

Objective： To explore the compatibility of hypaconitine（HA） and liquiritin, glycyrrhetinic acid on the detoxicated effect to myocardial ceils. Methods： Using the primary cultured myocardial cells as reseach subjects, to observe the changes of myocardial cells and vigour of superoxide dismutase （SOD）, glutathione peroxidase（GSH-PX） through liquiritin and glycyrrhetinic acid for HA. Results： By comparison with HA 120μmol/L, compatibility of HA 120μmol/L and liquirilin 480μmol/L with compatibility of HA 120μmol/L and glycyrrhetinic acid 120μmol/L increased vigour of SOD, GSH-PX, from （56.38±0.41）U/mL and （81.89±3.67）U/mgprot to （65.39±0.25）U/mL, （101.69±7.17）U/mgprot and （62.69±0.54）U/mL, （100.85±7.32）U/mgprot （P〈0.05）, compatibility of HA 120μmol/L and glycyrrhetinic acid 120μmol/L worked best （P〈0.01）. Conclusion： Compatibility of liquiritin 4800mol/L, glycyrrhetinic 1200rnol/L and HA1201amol/L can reduce the toxicity of HA to myocardial cells and increase vigor. There is a relationship between reducing oxidation and damaging of myocardial cells.